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. 2019 Jul 30:24:e00364.
doi: 10.1016/j.btre.2019.e00364. eCollection 2019 Dec.

Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor

Aline Alves Oliveira Santos Prado  1   2 , Brenda Lohanny Passos Santos  3 , Isabela Maria Monteiro Vieira  1   3 , Larissa Castor Ramos  1 , Roberto Rodrigues de Souza  1   3 , Daniel Pereira Silva  1   3 , Denise Santos Ruzene  1   3
Affiliations

Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor

Aline Alves Oliveira Santos Prado et al. Biotechnol Rep (Amst). .

Abstract

The biosurfactant production is characterized by high costs with substrates, which does not make them sufficiently competitive against synthetic surfactants. The insertion of alternative sources of low cost, especially agro-industrial residue, is an excellent alternative to make this competitiveness viable. An alkaline pretreatment was used to extract the hemicellulose from corncob in order to enhance its C5 fraction, common to vegetable biomasses. The hemicellulosic corncob liquor was used with glucose and mineral salt solution as carbon and nutrients sources in a fermentation process for the growth of Bacillus subtilis. It was performed a 23 full factorial design to determine the best conditions for the surfactin production in relation to the following response variables: surface tension reduction rate (STRR) and emulsification index (EI24), from which were obtained two optimized bioproducts under specific conditions. The optimized biosurfactants found to be effected presenting a critical micelle concentration of 100 mg.L-1 and a maximum bioremediation potential of 85.18%, as well as maximum values of 57.38% and 65.30% for STRR and EI24 variables, respectively. Overall results pointed for a successful commercial application for the surfactin produced.

Keywords: Bioremediation; Biosurfactant; Design of experiments; Hemicellulosic corncob liquor; Lignocellulosic residue.

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Figures

Fig. 1
Fig. 1
Three-dimensional response surface for surface tension reduction rate as a function of interaction between factors: (a) HCL and glucose and (b) HCL and mineral salt solution.
Fig. 2
Fig. 2
Three-dimensional response surface for emulsification index as a function of the interaction between factors (a) glucose and HCL and (b) mineral salt solution and HCL.
Fig. 3
Fig. 3
Plot of surface tension as a function of surfactin concentration to determine the value of CMC of the following emulsifier agents: (a) B-RTS, (b) B-EI24, (c) Glucose 4% and (d) surfactant Tween 80.
See this image and copyright information in PMC

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