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Comparative Study
. 1988 Dec 19;242(1):121-4.
doi: 10.1016/0014-5793(88)80998-0.

Characterization of the kinetics of neural cell adhesion molecule homophilic binding

N Moran  1 , E Bock
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Free article
Comparative Study

Characterization of the kinetics of neural cell adhesion molecule homophilic binding

N Moran et al. FEBS Lett. .
Free article

Abstract

A solid-phase assay has been developed for the investigation of the kinetics of neural cell adhesion molecule (NCAM) binding. Using this assay we can show that NCAM binds to itself in a time-dependent and saturable manner. Binding constants (KB values) of 6.9 x 10(-8) M and 1.23 x 10(-6) M, respectively, were obtained for adult and newborn rat NCAM homophilic binding. Binding is specifically inhibited by Fab' fragments of polyclonal anti-NCAM antibodies but is unaffected by heparin or chondroitin sulphate. This indicates that the NCAM homophilic binding site is separate from and independent of the heparin-binding site and that a developmental modification, probably polysialation, gives rise to marked differences in the adhesive properties of NCAM.

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