Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jan 15:303:125244.
doi: 10.1016/j.foodchem.2019.125244. Epub 2019 Jul 30.

UHPLC-MS/MS analysis of cocoa bean proteomes from four different genotypes

Emanuele Scollo  1 , David C A Neville  2 , M Jose Oruna-Concha  3 , Martine Trotin  2 , Rainer Cramer  4
Affiliations

UHPLC-MS/MS analysis of cocoa bean proteomes from four different genotypes

Emanuele Scollo et al. Food Chem. .

Abstract

In this study the proteomic profiles of cocoa beans from four genotypes with different flavour profiles were analysed by bottom-up label-free UHPLC-MS/MS. From a total of 430 identified proteins, 61 proteins were found significantly differentially expressed among the four cocoa genotypes analysed with a fold change of ≥2. PCA analysis allowed clear separation of the genotypes based on their proteomic profiles. Genotype-specific abundances were recorded for proteases involved in the degradation of storage proteins and release of flavour precursors. Different genotype-specific levels of other enzymes, which generate volatiles compounds that could potentially lead to flavour-inducing compounds, were also detected. Overall, this study shows that UHPLC-MS/MS data can differentiate cocoa bean varieties.

Keywords: Cocoa bean proteome; Cocoa beans; Cocoa flavour; Plant proteomics; Storage proteins; Theobroma cacao.

PubMed Disclaimer

LinkOut - more resources