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. 2019;14(11):1657339.
doi: 10.1080/15592324.2019.1657339. Epub 2019 Aug 26.

Different regulations of cell-type transcription by UV-B in multicellular green alga Volvox carteri

S Ekhtari  1 , J Razeghi  1 , K Hasanpur  2 , A Kianianmomeni  3
Affiliations

Different regulations of cell-type transcription by UV-B in multicellular green alga Volvox carteri

S Ekhtari et al. Plant Signal Behav. 2019.

Abstract

There is a scarcity of research reports on the effect of ultraviolet (UV)-B radiation on genome-wide transcriptional regulation in the multicellular green microalga including Volvox carteri (V. carteri). This microalga possesses only two cell types including mortal and motile somatic cells, as well as immortal and immotile reproductive cells. Therefore, the present study evaluated the effect of low-dose UV-B radiation on the cell-type-specific gene expression pattern of reproductive and somatic cells in an asexual life cycle of V. carteri using RNA sequence method. To this end, the separated reproductive and somatic cells were treated for 1 hour at an intensity of 0.056 mW/cm-2 UV-B radiation. Then, a transcriptome analysis was conducted between the UV-B and white light treated groups in either of the cell types. Based on differential gene expression analyses, no differentially expressed genes were found in reproductive cells under the treatment as compared to the control group. This type of cell maintained its steady state. However, treating the somatic cells with UV-B radiation led to at least 126 differentially expressed genes compared to the untreated control group. In addition, the results of a direct comparison demonstrated a restricted and wide response to UV-B radiation in somatic cells as compared to reproductive cells. Based on the results, UV-B radiation could be involved in cell-type-specific regulation of biological pathways.

Keywords: UV-B radiation. RNA sequence; Volvox carteri; gene regulatory; reproductive and somatic cells; transcriptomics.

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Figures

Figure 1.
Figure 1.
(a) The number of differentially up- and down-regulated gene features between treatment (UV-B) and control (WL) groups in somatic cells. Note. N is the total number of gene features with and New represents the number of unannotated gene features; (b) Differentially expressed genes (i.e., 0–1, 1–2, & >2 log2 fold change) in somatic cells.
Figure 2.
Figure 2.
The volcano plot of the transcriptome in somatic cells under UV-B and WL treatment. Note. The genes above the horizontal line are significantly based on a threshold (FDR<0.05) and the two-fold change threshold (|log2FoldChange|>1), respectively.
Figure 3.
Figure 3.
Heatmap (log RPKM+1) showing the expression profiles of differentially expressed genes between UV-B treated (UVB) and white light control (w) groups in somatic cells. Note. Due to non-stranded library to generate the data, some loci included two known genes that are marked with integer number postfixes on the graph. These loci are as below:
Figure 4.
Figure 4.
Functional analysis of DEGs between the UV-B treatment and control groups in the somatic cells based on gene ontology. GO category was presented for two major functional categories, namely biological process and molecular function.
See this image and copyright information in PMC

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