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. 2020 Jun;51(2):779-785.
doi: 10.1007/s42770-019-00145-8. Epub 2019 Aug 26.

The combined effect of high pressure processing and dimethyl dicarbonate to inactivate foodborne pathogens in apple juice

Rodrigo R Petrus  1 , John J Churey  2 , Gerard A Humiston  2 , Rebecca M Cheng  2 , Randy W Worobo  2
Affiliations

The combined effect of high pressure processing and dimethyl dicarbonate to inactivate foodborne pathogens in apple juice

Rodrigo R Petrus et al. Braz J Microbiol. 2020 Jun.

Abstract

Novel processing technologies can be used to improve both the microbiological safety and quality of food products. The application of high pressure processing (HPP) in combination with dimethyl dicarbonate (DMDC) represents a promising alternative to classical thermal technologies. This research work was undertaken to investigate the combined effect of HPP and DMDC, which was aimed at reaching over 5-log reduction in the reference pathogens Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes inoculated in apple juice. Different strains of each species were tested. The pressure (ranging from 100 to 600 MPa), dwell time (from 26 to 194 s), and DMDC (from 116 to 250 mg/L) were tested based on a central composite rotatable design. The dwell time, in the studied range, did not have a significant effect (p > 0.1) on the pathogens´ reduction. All treatments achieved a greater than 5-log reduction for E. coli O157:H7 and L. monocytogenes. The reductions for S. enterica were also greater than 5-log for almost all tested combinations. The results for S. enterica suggested that it is more resistant to HPP and DMDC compared with E. coli O157:H7 and L. monocytogenes. The findings of this study showed that DMDC at low concentrations can be added to apple juice to reduce the parameters conventionally applied in HPP. The combined use of HPP and DMDC was highly effective under the conditions of this study.

Keywords: Factorial design; Hurdle technology; Microbial inhibitor; Nonthermal processing.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Fig. 1
Fig. 1
Pareto diagram for reduction of S. enterica inoculated in apple juice (p ≤ 0.1). x1—pressure. x2—dwell time. x3—DMDC concentration
Fig. 2
Fig. 2
Response surface and contour curves in function of pressure and DMDC concentration for S. enterica reduction in apple juice
See this image and copyright information in PMC

References

    1. Khan I, Tango CN, Miskeen S, Lee BH, Oh DH. Hurdle technology: a novel approach for enhanced food quality and safety - a review. Food Control. 2017;73:1426–1444. doi: 10.1016/j.foodcont.2016.11.010. - DOI
    1. Singh S, Shalini R. Effect of hurdle technology in food preservation: a review. Crit Rev Food Sci Nutr. 2016;56(4):641–649. doi: 10.1080/10408398.2012.761594. - DOI - PubMed
    1. Balasubramaniam VM, Farkas D. High-pressure food processing. Food Sci Technol Int. 2018;14(5):413–418. doi: 10.1177/1082013208098812. - DOI
    1. Huang HW, Wu SJ, Lu JK, Shyu YT, Wang CY. Current status and future trends of high-pressure processing in food industry. Food Control. 2017;72:1–8. doi: 10.1016/j.foodcont.2016.07.019. - DOI
    1. Basaran-Akgul N, Churey JJ, Basaran P, Worobo RW. Inactivation of different strains of Escherichia coli O157:H7 in various apple ciders treated with dimethyl dicarbonate (DMDC) and sulfur dioxide (SO2) as an alternative method. Food Microbiol. 2009;26(1):8–15. doi: 10.1016/j.fm.2008.07.011. - DOI - PubMed

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