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. 2019 Apr 20;17(2):e1921.
doi: 10.21859/ijb.1921. eCollection 2019 Apr.

Integrin Beta-3 Gene Polymorphism and Risk for Myocardial Infarction in Premature Coronary Disease

Mehrdad Sheikhvatan  1 , Mohammad Ali Boroumand  1 , Mehrdad Behmanesh  2 , Shayan Ziaee  1 , Sara Cheraghee  1
Affiliations

Integrin Beta-3 Gene Polymorphism and Risk for Myocardial Infarction in Premature Coronary Disease

Mehrdad Sheikhvatan et al. Iran J Biotechnol. .

Abstract

Background: Contradictory results have been obtained regarding the role of integrin, beta 3 (ITGB3) gene polymorphisms in occurrence of myocardial infarction (MI).

Objectives: We aimed to assess the association between 1565C/T polymorphism of ITGB3 gene and increased risk for acute MI in patients with premature coronary artery disease (CAD).

Material and methods: Our study included 1000 premature CAD patients that classified into two groups with history of MI (n = 461) and without of MI (n = 539). The polymorphism variants in 10% of samples were determined by PCR-RFLP technique and genotyping of the polymorphism in all subjects was conducted by High Resolution Melting method. Given the two conditions of patients residing in Tehran and also faced with their first episode of MI, 640 out of 1000 study samples that had been previously followed-up were assessed in a retrospective cohort phase regarding long-term major adverse cardiac events (MACE).

Results: There was no significant difference in the frequency of 1565C/T polymorphism between the MI and non-MI groups. The frequency of wild genotype was 69.2% and 72.2%, the frequency of homozygous genotype was 21.3% and 18.4%, and the frequency of mutant genotype was 9.5% and 9.5%, respectively (P = 0.505). No significant difference was also found in total-MACE free survival rate between the patients with different genotypes of 1565C/T polymorphism in both MI and non-MI group.

Conclusions: The carriage of the 1565C/T polymorphism of ITGB3 gene seems unlikely to be a significant risk factor for the development of MI in Iranian patients with premature CAD.

Keywords: Atherosclerosis; Coronary Artery Disease; Genetics; Myocardial Infarction.

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Figures

Figure 1.
Figure 1.
Polymerase-chain-reaction (PCR) amplification of 1565C/T polymorphism of the ITGB3 gene (Agarose gel electrophoresis of DNA (2% agarose); PCR-based assay amplified a 331bp product, the 50-bp molecular size marker)
Figure 2.
Figure 2.
PCR-RFLP analysis with Msp I for 1565C/T polymorphism to determine polymorphism variants (Agarose gel electrophoresis of DNA (2% agarose); PCR-based assay amplified 331bp product and 252bp and 79bp products after effect of MspI enzyme with the restriction site of C^CGG, the 50-bp molecular size marker)
Figure 3.
Figure 3.
High resolution melting genotyping of the 1565C/T polymorphism
See this image and copyright information in PMC

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