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. 1988 Nov;214(3):592-4.
doi: 10.1007/BF00330500.

Functional expression of Aquaspirillum magnetotacticum genes in Escherichia coli K12

N S Waleh  1
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Functional expression of Aquaspirillum magnetotacticum genes in Escherichia coli K12

N S Waleh. Mol Gen Genet. 1988 Nov.

Abstract

Gene libraries from the magnetotactic bacterium, Aquaspirillum magnetotacticum were constructed in Escherichia coli with cosmids pLAFR3 and c2RB as vectors. Recombinant cosmids able to complement the thr-1, leuB, and proA mutations of the host were identified. The Pro+ recombinant cosmid restored wild-type phenotype in proA and proB but not in the proC mutants of E. coli. The results of restriction endonuclease digestion and Southern hybridization analysis indicate that the relevent leu and pro biosynthetic genes of A. magnetotacticum are not closely linked on the chromosome.

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References

    1. Appl Environ Microbiol. 1987 Dec;53(12):2733-8 - PubMed
    1. Arch Microbiol. 1978 Nov 13;119(2):203-12 - PubMed
    1. Microbiol Rev. 1983 Jun;47(2):180-230 - PubMed
    1. Mol Gen Genet. 1987 Jan;206(1):141-3 - PubMed
    1. Gene. 1983 Dec;26(2-3):137-46 - PubMed

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