Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jul 30;4(7):12865-12871.
doi: 10.1021/acsomega.9b00077. eCollection 2019 Jul 31.

Antimicrobial Activity of a New Class of Phosphorylated and Modified Flavonoids

Francis J Osonga  1 , Ali Akgul  2 , Roland M Miller  1 , Gaddi B Eshun  1 , Idris Yazgan  1 , Ayfer Akgul  2 , Omowunmi A Sadik  1
Affiliations

Antimicrobial Activity of a New Class of Phosphorylated and Modified Flavonoids

Francis J Osonga et al. ACS Omega. .

Abstract

The surge of resistant food pathogens is a major threat worldwide. Previous research conducted on phytochemicals has shown their antibacterial activity against pathogenic bacteria. The design of antimicrobial agents to curb pathogenic disease remains a challenge demanding critical attention. Flavonoids such as apigenin and quercetin were evaluated against Gram-positive and Gram-negative bacteria. The results indicated that the antibacterial activity of each flavonoid occurred at a different minimum inhibitory concentration. However, the antimicrobial activity results of the modified flavonoids were also reported, and it was observed that the Gram-positive bacteria were more susceptible in comparison to the Gram-negative bacteria. The cell wall structure of the Gram-positive and Gram-negative bacteria could be the main reason for the bacteria susceptibility. Modified flavonoids could be used as a suitable alternative antimicrobial agent for the treatment of infectious diseases. Our results indicated 100% inhibition of Listeria monocytogenes, Pseudomonas aeruginosa, and Aeromonas hydrophila with modified flavonoids.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
General structure of flavonoids showing the locations and identities of the various derivatives in the parent compounds.
Figure 2
Figure 2
Relative viability of L. monocytogenes after 16 h growth with test compounds. The error bars show +1 standard deviation for each measurement with three replicates. *p < 0.05. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed using the GraphPad Prism 8.0, GraphPad Software.
Figure 3
Figure 3
Relative viability of A. hydrophila after 16 h growth with test compounds. The error bars showed +1 standard deviation for each measurement with three replicates. *p < 0.0001. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed using the GraphPad Prism, 8.0 GraphPad Software.
Figure 4
Figure 4
Relative viability of P. aeruginosa after 16 h growth with test compounds. The error bars showed +1 standard deviation for each measurement with three replicates. *p < 0.0001. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed using the GraphPad Prism, 8.0 GraphPad Software.
Figure 5
Figure 5
Viable counting for A. hydrophila on a non-molecule-treated plate with the 10–5 dilution of control and 0.35 mg/mL of QCR-treated LB broth. Each plate is a different replicate.
Figure 6
Figure 6
Viable counting for P. aeruginosa on a non-molecule-treated plate with the 10–5 dilution of control and 0.35 mg/mL of QCR-treated LB-broth. Each plate is a different replicate.
Figure 7
Figure 7
Viable counting for L. monocytogenes on a non-molecule-treated plate with the 10–5 dilution of control and 0.35 mg/mL of QCR-treated LB-broth. Total covered area by L. monocytogenes was decreased over 95%. Each plate is a different replicate.
See this image and copyright information in PMC

References

    1. Ahmad A.; Kaleem M.; Ahmed Z.; Shafiq H. Therapeutic potential of flavonoids and their mechanism of action against microbial and viral infections—A review. Food Res. Int. 2015, 77, 221–235. 10.1016/j.foodres.2015.06.021. - DOI
    1. Nakamura K.; Ishiyama K.; Sheng H.; Ikai H.; Kanno T.; Niwano Y. Bactericidal activity and mechanism of photoirradiated polyphenols against Gram-positive and-negative bacteria. J. Agric. Food Chem. 2015, 63, 7707–7713. 10.1021/jf5058588. - DOI - PubMed
    1. Daglia M. Polyphenols as antimicrobial agents. Curr. Opin. Biotechnol. 2012, 23, 174–181. 10.1016/j.copbio.2011.08.007. - DOI - PubMed
    1. Mandalari G.; Bennett R. N.; Bisignano G.; Trombetta D.; Saija A.; Faulds C. B.; Gasson M. J.; Narbad A. Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamia Risso) peel, a byproduct of the essential oil industry. J. Appl. Microbiol. 2007, 103, 2056–2064. 10.1111/j.1365-2672.2007.03456.x. - DOI - PubMed
    1. Wu T.; Li H.; Chen J. C.; Cao Y.; Fu W.; Zhou P.; Pang J. Apigenin, a novel candidate involving herb-drug interaction (HDI), interacts with organic anion transporter 1 (OAT1). Pharmacol. Rep. 2017, 69, 1254–1262. 10.1016/j.pharep.2017.06.012. - DOI - PubMed

LinkOut - more resources