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Multicenter Study
. 2019 Aug 23:8:143.
doi: 10.1186/s13756-019-0576-5. eCollection 2019.

Antimicrobial susceptibility and molecular characteristics of Mycoplasma pneumoniae isolates across different regions of China

Fei Zhao #  1 , Jing Li #  2 , Jinrong Liu  3 , Xuemei Guan  4 , Jie Gong  1 , Liyong Liu  1 , Lihua He  1 , Fanliang Meng  1 , Jianzhong Zhang  1
Affiliations
Multicenter Study

Antimicrobial susceptibility and molecular characteristics of Mycoplasma pneumoniae isolates across different regions of China

Fei Zhao et al. Antimicrob Resist Infect Control. .

Abstract

Background: In China mainland, most Mycoplasma pneumoniae related studies are carried out in Beijing and Shanghai, while rare studies are performed in the other regions. In this study, we analyzed the molecular biology characteristics and antimicrobial susceptibility of clinical isolates of M. pneumoniae from 5 regions between January 2017 and December 2018.

Methods: Genotyping was performed to 154 M. pneumoniae isolates from 5 cities using PCR and multiple-locus variable-number tandem repeat analysis (MLVA) method. Antimicrobial susceptibility test was performed to all the isolates against 4 antibiotics. Sequencing was performed to the amplification products of the 23S rRNA drug resistant gene.

Results: Genotype I was detected in 118 M. pneumoniae isolates (76.6%), and genotype II was identified in 36 isolates (23.4%). The majority (92.2%) of the MLVA genotypes were 4-5-7-2 and 3-5-6-2, which represented the genotype I and II, respectively. The total macrolide (ML) resistance rate was 79.7%. The minimum inhibitory concentration (MIC) of the erythromycin was in a range of 128- > 256 μg/ml, while that for the azithromycin was 2-32 μg/ml. There were mutations in the 23S rRNA in each ML resistance isolate. Jilin city showed the highest prevalence of genotype I (100%) and ML resistance rate (100%), while Jinan showed the lowest prevalence of genotype I (45.5%) and ML resistance rate (54.5%).

Conclusions: A large variance was identified in the M. pneumoniae genotype and ML resistance among the 5 cities. The proportion of M. pneumoniae with a genotype II genotype (3-5-6-2) showed an increased trend.

Keywords: 23S rRNA gene; Genotype; Macrolide resistance; Mycoplasma pneumoniae.

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Conflict of interest statement

Competing interestsThe authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Locality map, genotype and ML resistant rate of five cities included in this study
Fig. 2
Fig. 2
Locality map of ten cities included in this study(●) and newly publication(▲ Ref [21]). The genotyping and ML resistance were nearly the same in the cities in the cycle
See this image and copyright information in PMC

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