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. 2020 Jan;101(1):71-86.
doi: 10.1111/tpj.14518. Epub 2019 Oct 14.

The H3 histone chaperone NASPSIM3 escorts CenH3 in Arabidopsis

Samuel Le Goff  1 , Burcu Nur Keçeli  2 , Hana Jeřábková  3 , Stefan Heckmann  4 , Twan Rutten  4 , Sylviane Cotterell  1 , Veit Schubert  4 , Elisabeth Roitinger  5   6   7 , Karl Mechtler  5   6   7 , F Christopher H Franklin  8 , Christophe Tatout  1 , Andreas Houben  4 , Danny Geelen  2 , Aline V Probst  1 , Inna Lermontova  4   9
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Free article

The H3 histone chaperone NASPSIM3 escorts CenH3 in Arabidopsis

Samuel Le Goff et al. Plant J. 2020 Jan.
Free article

Abstract

Centromeres define the chromosomal position where kinetochores form to link the chromosome to microtubules during mitosis and meiosis. Centromere identity is determined by incorporation of a specific histone H3 variant termed CenH3. As for other histones, escort and deposition of CenH3 must be ensured by histone chaperones, which handle the non-nucleosomal CenH3 pool and replenish CenH3 chromatin in dividing cells. Here, we show that the Arabidopsis orthologue of the mammalian NUCLEAR AUTOANTIGENIC SPERM PROTEIN (NASP) and Schizosaccharomyces pombe histone chaperone Sim3 is a soluble nuclear protein that binds the histone variant CenH3 and affects its abundance at the centromeres. NASPSIM3 is co-expressed with Arabidopsis CenH3 in dividing cells and binds directly to both the N-terminal tail and the histone fold domain of non-nucleosomal CenH3. Reduced NASPSIM3 expression negatively affects CenH3 deposition, identifying NASPSIM3 as a CenH3 histone chaperone.

Keywords: Arabidopsis thaliana; CenH3; NASPSIM3; centromere; histone chaperone; kinetochore.

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References

    1. Apta-Smith, M.J., Hernandez-Fernaud, J.R. and Bowman, A.J. (2018) Evidence for the nuclear import of histones H3.1 and H4 as monomers. EMBO J. 37, e98714.
    1. Barnhart, M.C., Kuich, P.H.J.L., Stellfox, M.E., Ward, J.A., Bassett, E.A., Black, B.E. and Foltz, D.R. (2011) HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore. J. Cell Biol. 194, 229-243.
    1. Batzenschlager, M., Masoud, K., Janski, N. et al. (2013) The GIP gamma-tubulin complex-associated proteins are involved in nuclear architecture in Arabidopsis thaliana. Front. Plant Sci. 4, 480.
    1. Batzenschlager, M., Lermontova, I., Schubert, V. et al. (2015) Arabidopsis MZT1 homologs GIP1 and GIP2 are essential for centromere architecture. Proc. Natl Acad. Sci. USA, 112, 8656-8660.
    1. Benoit, M., Simon, L., Desset, S., Duc, C., Cotterell, S., Poulet, A., Goff, S.Le, Tatout, C. and Probst, A.V. (2019) Replication-coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development. New Phytol. 221, 385-398.

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