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. 2019 Oct;16(10):e1900341.
doi: 10.1002/cbdv.201900341. Epub 2019 Sep 18.

Phytochemical Content, Antidiabetic, Anticholinergic, and Antioxidant Activities of Endemic Lecokia cretica Extracts

Abdülmelik Aras  1 , Ercan Bursal  2 , Fikret Türkan  3 , Hatice Tohma  4 , Ömer Kılıç  5 , İlhami Gülçin  6 , Ekrem Köksal  4
Affiliations

Phytochemical Content, Antidiabetic, Anticholinergic, and Antioxidant Activities of Endemic Lecokia cretica Extracts

Abdülmelik Aras et al. Chem Biodivers. 2019 Oct.

Abstract

The aim of this work was to investigate the enzyme inhibition, antioxidant activity, and phenolic compounds of Lecokia cretica (Lam.) DC. Acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glycosidase enzymes were strongly inhibited by the L. cretica extracts. IC50 values for the three enzymes were found as 3.21 mg/mL, 2.1 mg/mL, and 2.07 mg/mL, respectively. Antioxidant activities were examined in both aqueous and ethanol (EtOH) extracts using CUPRAC, FRAP, and DPPH method. Also, the phenolic compounds of the endemic plant were identified and quantified by using HPLC/MS/MS. According to the results, the extracts have remarkable antioxidant activities. The most abundant phenolic acids of L. cretica in EtOH extract were determined as quinic acid (12.76 mg/kg of crude extract), chlorogenic acid (3.39 mg/kg), and malic acid (2.38 mg/kg).

Keywords: HPLC/MS/MS; Lecokia cretica; anticholinergic activity; antidiabetic activity; antioxidant activity.

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