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. 2019 Aug 28;9(9):1215.
doi: 10.3390/nano9091215.

Biocompatibility of Cyclopropylamine-Based Plasma Polymers Deposited at Sub-Atmospheric Pressure on Poly (ε-caprolactone) Nanofiber Meshes

Ke Vin Chan  1 , Mahtab Asadian  2 , Iuliia Onyshchenko  2 , Heidi Declercq  3 , Rino Morent  2 , Nathalie De Geyter  2
Affiliations

Biocompatibility of Cyclopropylamine-Based Plasma Polymers Deposited at Sub-Atmospheric Pressure on Poly (ε-caprolactone) Nanofiber Meshes

Ke Vin Chan et al. Nanomaterials (Basel). .

Abstract

In this work, cyclopropylamine (CPA) monomer was plasma-polymerized on poly (ε-caprolactone) nanofiber meshes using various deposition durations to obtain amine-rich surfaces in an effort to improve the cellular response of the meshes. Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) were used to investigate the surface morphology and surface chemical composition of the PCL samples, respectively. The measured coating thickness was found to linearly increase with deposition duration at a deposition rate of 0.465 nm/s. XPS analysis revealed that plasma exposure time had a considerable effect on the surface N/C and O/C ratio as well as on amino grafting efficiency and amino selectivity. In addition, cell studies showed that cell adhesion and proliferation significantly improved for all coated samples.

Keywords: biomaterials; cell adhesion; nanofibers; non-thermal plasma; plasma polymerisation.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
SEM images of (a) untreated and (b–h) PPF-NFs with various deposition durations: (b) 10 s, (c) 20 s, (d) 60 s, (e) 90 s, (f) 120 s, (g) 240 s and (h) 360 s.
Figure 2
Figure 2
The evolution of the plasma-polymerized film thickness with plasma deposition duration.
Figure 3
Figure 3
Evolution of the N/C and O/C ratio as a function of plasma deposition duration.
Figure 4
Figure 4
Evolution of amino grafting efficiency (NH2/C) and amino selectivity (NH2/N) as a function of plasma deposition duration.
Figure 5
Figure 5
The deconvolution of XPS high resolution C1s peaks of untreated PCL NFs and PPF-NFs with a deposition duration varying between 10 and 360 s.
Figure 6
Figure 6
Changes in chemical bond concentrations between untreated PCL NFs and PPF-NFs as a function of plasma deposition duration.
Figure 7
Figure 7
Fluorescence and SEM images of HFFs adhering on (a) untreated PCL NFs and (b–h) PPFs prepared using various deposition durations ((b) 10 s, (c) 20 s, (d) 60 s, (e) 90 s, (f) 120 s, (g) 240 s and (h) 360 s) 1 day after cell seeding.
Figure 8
Figure 8
Fluorescence and SEM images of HFFs adhering on (a) untreated PCL NFs and (b–h) PPFs prepared using various deposition durations ((b) 10 s, (c) 20 s, (d) 60 s, (e) 90 s, (f) 120 s, (g) 240 s and (h) 360 s) 7 days after cell seeding.
Figure 9
Figure 9
Cell viability 1 day and 7 days after cell seeding on pristine PCL NFs and PPF-NFs prepared using different plasma deposition times. The results are presented relative to the viability of TCPS on day 7 (* denotes statistical significance at * p < 0.05, ** p < 0.005).
Figure 10
Figure 10
Evolution of the average shape factor of the adherent cells for the untreated and PPF-NFs with different deposition durations on day 1.
See this image and copyright information in PMC

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