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. 2019 Aug 28;9(8):e028866.
doi: 10.1136/bmjopen-2018-028866.

The Uppsala-Stockholm Assisted Reproductive Techniques (UppStART) study

Anastasia N Iliadou  1 , Anna Sara Öberg  1   2 , Jessica Pege  1 , Kenny A Rodriguez-Wallberg  3   4 , Jan I Olofsson  5 , Jan Holte  6   7   8 , Håkan Wramsby  9 , Margaretha Wramsby  10 , Sven Cnattingius  11 , Carolyn E Cesta  12   13
Affiliations

The Uppsala-Stockholm Assisted Reproductive Techniques (UppStART) study

Anastasia N Iliadou et al. BMJ Open. .

Abstract

Purpose: The Uppsala-Stockholm Assisted Reproductive Techniques (UppStART) study is a prospectively recruited sample of couples undergoing assisted reproduction in Stockholm and Uppsala county in Sweden. The study was initiated to (1) investigate possible changes in the epigenetic profile of infants inferred through the ART procedures and their consequence and (2) to assess the impact of lifestyle and health exposures on treatment outcome.

Participants: Recruitment took place between September 2011 and December 2013, and in vitro fertilisation (IVF) cycles initiated and pregnancies conceived during this time were followed until December 2014. The cohort includes 971 participants (n= 514 women; n= 457 men), and 129 pregnancies were achieved from the first IVF cycle included in the study.

Findings to date: Self-reported demographic, health and lifestyle data were collected from a baseline questionnaire, and to assess changes to lifestyle, a follow-up questionnaire was issued at the time of oocyte retrieval, and at subsequent IVF cycles. Questionnaire data were linked to data extracted from medical records. Biological samples were collected at baseline: blood for extraction of serum, plasma and DNA, morning and evening saliva samples for cortisol measurement and at delivery including samples of maternal blood, placenta and amniotic fluid, and cord blood for epigenetic analysis.

Future plans: Through the unique identification number assigned to each Swedish citizen at birth or immigration, UppStART study participants will be linked to the Swedish population-based national and quality registers to provide data from prenatal, obstetrical, neonatal and infant care, and subsequent updates will provide data on childhood health and educational outcomes. Collaboration and use of UppStART data is encouraged, and more information about access can be found at www.ki.se/meb/uppstart.

Keywords: assisted reproductive techniques; epigenetics; in vitro fertilisation; infertility; lifestyle.

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Conflict of interest statement

Competing interests: None declared.

Figures

Figure 1
Figure 1
Timeline of participant recruitment and data collection in the Uppsala–Stockholm Assisted Reproductive Techniques study. ART, assistedreproductive techniques; ICSI, intracytoplasmic sperm injection; IVF, in vitro fertilisation.
Figure 2
Figure 2
Uppsala–Stockholm Assisted Reproductive Techniques study: number of participants and samples collected. IVF, in vitro fertilisation.
See this image and copyright information in PMC

References

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