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. 2018;31(5):3-8.
doi: 10.1080/08940886.2018.1506233. Epub 2018 Sep 25.

Microscopy Instrumentation and Nanopositioning at NSLS-II: Current Status and Future Directions

E Nazaretski  1 , W Xu  1 , H Yan  1 , X Huang  1 , D S Coburn  1 , M Ge  1 , W-K Lee  1 , Y Gao  1 , W Xu  1 , M Fuchs  1 , Y S Chu  1
Affiliations

Microscopy Instrumentation and Nanopositioning at NSLS-II: Current Status and Future Directions

E Nazaretski et al. Synchrotron Radiat News. 2018.
No abstract available

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Figures

Figure 1:
Figure 1:
Two types of nano-focusing optics implemented in the HXN microscope: (a) MLLs; (b) ZP optics. For MLLs, two linear optics have to be aligned orthogonally with respect to each other to achieve point focusing. The degrees of motion required to perform optics alignment and manipulation are shown.
Figure 2:
Figure 2:
(a) Photograph of the MLL-based microscope installed in the experimental hutch of the HXN beamline; 1–granite support and the microscope vacuum chamber; 2–granite support and the fluorescence detector; (b) CAD model of the MLL/ZP modules installed inside the vacuum chamber; (c) inside view of the vacuum chamber from the downstream side, where MLL and ZP modules are staggered; (d) photograph of the MLL module; 1–the vertical MLL assembly; 2–horizontal MLL assembly; 3–OSA motion stack; 4–sample stage; 5–fiber optics interferometers; (e) photograph of the ZP module; 1–sample stage; 2–ZP carriage; 3–OSA assembly; 4–fiber optic interferometers.
Figure 3:
Figure 3:
(a) 0.5 nm steps recorded by the interferometer during evaluation of the MLL module in the nano-positioning laboratory; solid line represents the trajectory of motion; (b) FFT spectrum of the sample stage in the y-direction; measurements were taken at the HXN beamline after the installation of the MLL module inside the vacuum chamber; fundamental resonance frequencies are well above 100 Hz; (c) XRF image of a Pt test pattern (L-edge, 12 keV photon energy, exposure time 0.2 s, 5 nm per pixel); diameter of the rings is 80 nm, line width 20 nm, and the height is 200 nm.
Figure 4:
Figure 4:
(a) Schematic of TXM; b) photograph of the TXM system installed in the experimental hutch; 1–TXM microscope; 2–detector setup; (c) photograph of the TXM sample area; 1–sample stage assembly; 2–zone plate assembly; 3–pinhole support; 4–capillary condenser manipulator; (d) 3D reconstruction of an iron-cupper (Fe-Cu) sample, pixel size 30 nm; (e) closer look at one of the tomograms.
Figure 5:
Figure 5:
(a) The FastForward MX goniometer system installed in the FMX experimental station. (b) Schematic of the piezo scanner. P1, P2, and P3 are the motion directions of three shear piezo actuators of the NanoCube scanner. Yc and Zc are the motions of the NEXLINE coarse stages. Both fine and coarse stages are mounted on top of an air-bearing rotational stage, Ω. (c) Scanning trajectory measured in the x-direction at 40 Hz scanning frequency (blue solid line). The red dashed line demonstrates the selected trajectory. (d) Raster scan of 5- to 10-μm-sized proteinase K crystals loaded on a MiTeGen loop. The raster scan was performed as a fly-scan with 2 μm translation and 2.5 ms exposure time per frame (i.e., scanning frequency of 10 linepairs/s). The heatmap encodes the diffraction pattern spot count. The scan area is shown as a red-dashed rectangle in the inset.
See this image and copyright information in PMC

References

    1. Kang HC et al., Nanoscale Communication 5, 7184 (2013). - PubMed
    1. Yan H et al., Scientific Reports 6, 20112 (2016). - PMC - PubMed
    1. Holler M et al., Nature 543, 402 (2017). - PubMed
    1. Hill MO et al., Nano Letters 18(2), 811 (2018). - PubMed
    1. Kang HC et al., Nanoscale Communication 5, 7184 (2013). - PubMed

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