Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Dec;54(12):2933-7.
doi: 10.1128/aem.54.12.2933-2937.1988.

Synthetic oligodeoxyribonucleotide probes for detection of Listeria monocytogenes

A R Datta  1 , B A Wentz, D Shook, M W Trucksess
Affiliations

Synthetic oligodeoxyribonucleotide probes for detection of Listeria monocytogenes

A R Datta et al. Appl Environ Microbiol. 1988 Dec.

Abstract

A 500-base-pair DNA fragment of a presumptive beta-hemolysin gene of Listeria monocytogenes has been used to identify this organism by a modified colony hybridization technique. We have cloned this DNA fragment into M13 bacteriophage vectors and sequenced it by a dideoxynucleotide sequencing technique. From this sequencing information, several oligodeoxyribonucleotides were synthesized and used as synthetic probes to identify L. monocytogenes. The probes were specific for L. monocytogenes and did not react with any other Listeria strains in a colony hybridization assay. In particular, one of these probes (AD07) was used to detect L. monocytogenes in artificially contaminated raw-milk and soft-cheese samples.

PubMed Disclaimer

References

    1. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed
    1. N Engl J Med. 1983 Jan 27;308(4):203-6 - PubMed
    1. Bacteriol Rev. 1966 Jun;30(2):309-82 - PubMed
    1. Arch Intern Med. 1986 Mar;146(3):520-4 - PubMed
    1. Infect Immun. 1986 Jan;51(1):314-9 - PubMed

MeSH terms

LinkOut - more resources