Temperature variation magnifies chlorpyrifos toxicity differently between larval and adult mosquitoes

Abstract

To improve risk assessment there is increasing attention for the effect of climate change on the sensitivity to contaminants and vice versa. Two important and connected topics have been largely ignored in this context: (i) the increase of daily temperature variation (DTV) as a key component of climate change, and (ii) differences in sensitivity to climate change and contaminants between developmental stages. We therefore investigated whether DTV magnified the negative effects of the organophosphate insecticide chlorpyrifos on mortality and heat tolerance and whether this effect was stronger in aquatic larvae than in terrestrial adults of the mosquito Culex pipiens. Exposure to chlorpyrifos at a constant temperature imposed mortality and reduced the heat tolerance in both larvae and adult males, but not in adult females. This provides the first evidence that the TICS ("toxicant-induced climate change sensitivity") concept can be sex-specific. DTV had no direct negative effects. Yet, consistent with the CITS ("climate-induced toxicant sensitivity") concept, DTV magnified the toxicity of the pesticide in terms of larval mortality. This was not the case in the adult stage indicating the CITS concept to be dependent on the developmental stage. Notably, chlorpyrifos reduced the heat tolerance of adult females only in the presence of DTV, thereby providing support for the reciprocal effects between DTV and contaminants, hence the coupling of the TICS and CITS concepts. Taken together, our results highlight the importance of integrating DTV and the developmental stage to improve risk assessment of contaminants under climate change.

Keywords: Complex life cycle; Daily temperature fluctuation; Global warming; Multistressor; Pesticide; Upper thermal tolerance.