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Review
. 2019 Sep;16(9):830-842.
doi: 10.1038/s41592-019-0538-0. Epub 2019 Aug 30.

Biological imaging of chemical bonds by stimulated Raman scattering microscopy

Fanghao Hu #  1 , Lixue Shi #  1 , Wei Min  2   3
Affiliations
Review

Biological imaging of chemical bonds by stimulated Raman scattering microscopy

Fanghao Hu et al. Nat Methods. 2019 Sep.

Abstract

All molecules consist of chemical bonds, and much can be learned from mapping the spatiotemporal dynamics of these bonds. Since its invention a decade ago, stimulated Raman scattering (SRS) microscopy has become a powerful modality for imaging chemical bonds with high sensitivity, resolution, speed and specificity. We introduce the fundamentals of SRS microscopy and review innovations in SRS microscopes and imaging probes. We highlight examples of exciting biological applications, and share our vision for potential future breakthroughs for this technology.

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