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. 2019 Oct;50(4):1063-1073.
doi: 10.1007/s42770-019-00133-y. Epub 2019 Sep 2.

Prevalence, virulence characterization, and genetic relatedness of Listeria monocytogenes isolated from chicken retail points and poultry slaughterhouses in Turkey

Aysen Coban  1   2 , Vincenzo Pennone  1 , Mert Sudagidan  3 , Celenk Molva  4 , Kieran Jordan  1 , Ali Aydin  5
Affiliations

Prevalence, virulence characterization, and genetic relatedness of Listeria monocytogenes isolated from chicken retail points and poultry slaughterhouses in Turkey

Aysen Coban et al. Braz J Microbiol. 2019 Oct.

Abstract

Listeria monocytogenes is one of the most important foodborne pathogens and is a causal agent of listeriosis in humans and animals. The aim of this study was to determine the prevalence, serogroups, antibiotic susceptibility, virulence factor genes, and genetic relatedness of L. monocytogenes strains isolated from 500 poultry samples in Turkey. The isolation sources of 103 L. monocytogenes strains were retail markets (n = 100) and slaughterhouses (n = 3). L. monocytogenes strains were identified as serogroups 1/2a-3a (75.7%, lineage I), 1/2c-3c (14.56%, lineage I), 1/2b-3b-7 (5.82%, lineage II), 4a-4c (2.91%, lineage III), and 4b-4d-4e (0.97%, lineage III). Most of the L. monocytogenes strains (93.2%) were susceptible to the antibiotics tested. PCR analysis indicated that the majority of the strains (95% to 100%) contained most of the virulence genes (hylA, plcA, plcB, prfA, mpl, actA, dltA, fri, flaA inlA, inlC, and inlJ). Pulsed-field gel electrophoresis (PFGE) demonstrated that there were 18 pulsotypes grouped at a similarity of > 90% among the strains. These results indicate that it is necessary to prevent the presence of L. monocytogenes in the poultry-processing environments to help prevent outbreaks of listeriosis and protect public health.

Keywords: Antibiotic susceptibility; Chicken meat; Listeria monocytogenes; PFGE; Virulence characterization.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Dendrogram showing PFGE band patterns of 103 L. monocytogenes strains divided into 18 pulsotypes after AscI and ApaI digestion
Fig. 2
Fig. 2
Minimum spanning tree obtained from dendrogram analysis of all the isolates obtained from the survey. The white circles represent pulsotypes with no similarity at < 90%, while the colors indicate pulsotypes with > 90% similarity. Pulsotypes 1, 4, and 12 contain isolates with mixed serogroups
Fig. 3
Fig. 3
Comparison of some PFGE profiles of different isolates showing the widespread nature of the isolates (a), the persistent nature of isolates (b), and isolates of different serogroups in the same pulsotype (c). Isolation sources: M1, Habibler (Istanbul); M2, Taksim (Istanbul); M3, Avcilar (Istanbul); M4, Sehremini (Istanbul); M5, Fatih (Istanbul); S1, Slaughterhouse A (Bolu)
Fig. 4
Fig. 4
Comparison PFGE profiles of L. monocytogenes strains isolated from Turkey (Tr-coded strains) with isolates from Ireland
See this image and copyright information in PMC

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