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. 1978;6(1):13-21.

[Separation of lymphocytes into classes from peripheral human blood using centrifugation on a discontinuous gradient]

[Article in Croatian]
M Hadzija, M Poljak-Blazi, M Boranić
  • PMID: 314801

[Separation of lymphocytes into classes from peripheral human blood using centrifugation on a discontinuous gradient]

[Article in Croatian]
M Hadzija et al. Bilt Hematol Transfuz. 1978.

Abstract

Peripheral blood lymphocytes of 20 normal volunteers were separated on a discontinuous gradient of Ficoll. The ability of lymphocytes from the fractions to from sponteneous (E) and induced (EAC) rosettes and undergo transformation in the presence of phytohaemagglutinin (PHA) was tested. T-lymphocytes were concentrated in the density of 1,067 g/ml and B-lymphocytes in the density of 1,063 g/ml.

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