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. 2019;72(2):142-148.
doi: 10.5173/ceju.2019.1949. Epub 2019 May 6.

Effects of intravesical administration of sensory neuron-specific receptor agonist on voiding function in rats with cyclophosphamide-induced cystitis

Masashi Honda  1 , Panagiota Tsounapi  1 , Shogo Teraoka  1 , Yusuke Kimura  1 , Katsuya Hikita  1 , Atsushi Takenaka  1
Affiliations

Effects of intravesical administration of sensory neuron-specific receptor agonist on voiding function in rats with cyclophosphamide-induced cystitis

Masashi Honda et al. Cent European J Urol. 2019.

Abstract

Introduction: The aim of this study was to investigate the urodynamic effects of intravesical administration of bovine adrenal medulla 8-22 (BAM8-22), a selective rat sensory neuron-specific receptor 1 agonist, on the micturition reflex in normal rats and rats with cyclophosphamide-induced bladder overactivity.

Material and methods: Continuous cystometrograms (0.04 ml/min) were performed in urethane-anesthetized rats. After stable micturition cycles were established, vehicle (saline) or BAM8-22 was instilled intravesically and changes in bladder activity were monitored. The experiments using BAM8-22 were also performed in capsaicin-pretreated rats. In another experiment, vehicle (saline) or BAM8-22 was instilled intravesically and changes in bladder activity were monitored in cyclophosphamide-treated rats. Continuous cystometrograms were performed 48 hours after cyclophosphamide injection. Cystometric parameters were recorded and compared before and after intravesical drug administration.

Results: Intravesical administration of BAM8-22 significantly increased the intercontraction interval and threshold pressure in urethane-anesthetized rats, but did not affect the basal pressure or maximum pressure at any doses tested. The inhibitory effects of intravesical administration of BAM8-22 were not inhibited by capsaicin pretreatment. Intravesical administration of BAM8-22 also significantly increased intercontraction interval in the cyclophosphamide-treated rats.

Conclusions: The current results indicate that intravesical administration of a selective rat sensory neuron-specific receptor 1 agonist can inhibit the micturition reflex and can ameliorate cyclophosphamide-induced bladder overactivity in rats.

Keywords: cyclophosphamide; cystometry; rats; sensory neuron-specific receptor.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Representative cystometrograms showing the effects of intravesical administration of BAM8-22 (1000 nM) on bladder activity in normal rats. B. Representative cystometrograms showing the effects of intravesical administration of BAM8-22 (1000 nM) on bladder activity in capsaicin-pretreated rats.
Figure 2
Figure 2
Representative cystometrograms showing the effects of intravesical administration of BAM8-22 (3000 nM) on bladder activity in cyclophosphamide-treated rats.
Figure 3
Figure 3
Changes in the intercontraction interval (ICI) (% of control [Pre]) after intravesical administration of vehicle or BAM8-22 in cyclophosphamide-treated rats. Histograms represent mean ± standard deviation values. *<0.01 vs. vehicle administration
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