Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988;54(6):567-73.
doi: 10.1007/BF00588392.

Low- and intermediate-copy-number cloning vectors based on the Pseudomonas plasmid pVS1

Y Itoh  1 , L Soldati, T Leisinger, D Haas
Affiliations

Low- and intermediate-copy-number cloning vectors based on the Pseudomonas plasmid pVS1

Y Itoh et al. Antonie Van Leeuwenhoek. 1988.

Abstract

The cloning vector pME290 (6.8 kb), which is derived from the Pseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1-3 copies) and pME294 (about 15-20 copies) were isolated. These vectors were used in the characterization of the P. aeruginosa argF gene encoding ornithine carbamoyltransferase.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Gene. 1981 Dec;16(1-3):227-35 - PubMed
    1. Mol Gen Genet. 1986 Jun;203(3):421-9 - PubMed
    1. Antimicrob Agents Chemother. 1980 Feb;17(2):165-9 - PubMed
    1. Science. 1987 Jan 30;235(4788):587-91 - PubMed
    1. Mol Gen Genet. 1976 Apr 23;145(1):101-8 - PubMed

Publication types

LinkOut - more resources