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. 1988;54(6):567-73.
doi: 10.1007/BF00588392.

Low- and intermediate-copy-number cloning vectors based on the Pseudomonas plasmid pVS1

Y Itoh  1 , L Soldati, T Leisinger, D Haas
Affiliations

Low- and intermediate-copy-number cloning vectors based on the Pseudomonas plasmid pVS1

Y Itoh et al. Antonie Van Leeuwenhoek. 1988.

Abstract

The cloning vector pME290 (6.8 kb), which is derived from the Pseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1-3 copies) and pME294 (about 15-20 copies) were isolated. These vectors were used in the characterization of the P. aeruginosa argF gene encoding ornithine carbamoyltransferase.

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