Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Oct;31(42):e1903599.
doi: 10.1002/adma.201903599. Epub 2019 Sep 5.

Narrowband Organic Light-Emitting Diodes for Fluorescence Microscopy and Calcium Imaging

Caroline Murawski  1 , Andreas Mischok  1 , Jonathan Booth  1   2 , Jothi Dinesh Kumar  1 , Emily Archer  1 , Laura Tropf  1 , Chang-Min Keum  1 , Ya-Li Deng  1 , Kou Yoshida  1 , Ifor D W Samuel  1 , Marcel Schubert  1 , Stefan R Pulver  2 , Malte C Gather  1
Affiliations

Narrowband Organic Light-Emitting Diodes for Fluorescence Microscopy and Calcium Imaging

Caroline Murawski et al. Adv Mater. 2019 Oct.

Abstract

Fluorescence imaging is an indispensable tool in biology, with applications ranging from single-cell to whole-animal studies and with live mapping of neuronal activity currently receiving particular attention. To enable fluorescence imaging at cellular scale in freely moving animals, miniaturized microscopes and lensless imagers are developed that can be implanted in a minimally invasive fashion; but the rigidity, size, and potential toxicity of the involved light sources remain a challenge. Here, narrowband organic light-emitting diodes (OLEDs) are developed and used for fluorescence imaging of live cells and for mapping of neuronal activity in Drosophila melanogaster via genetically encoded Ca2+ indicators. In order to avoid spectral overlap with fluorescence from the sample, distributed Bragg reflectors are integrated onto the OLEDs to block their long-wavelength emission tail, which enables an image contrast comparable to conventional, much bulkier mercury light sources. As OLEDs can be fabricated on mechanically flexible substrates and structured into arrays of cell-sized pixels, this work opens a new pathway for the development of implantable light sources that enable functional imaging and sensing in freely moving animals.

Keywords: OLED; calcium imaging; distributed Bragg reflector; fluorescence microscopy.

PubMed Disclaimer

References

    1. P. R. Roelfsema, D. Denys, P. C. Klink, Trends Cognit. Sci. 2018, 22, 598.
    1. T. H. Kim, Y. Zhang, J. Lecoq, J. C. Jung, J. Li, H. Zeng, C. M. Niell, M. J. Schnitzer, Cell Rep. 2016, 17, 3385.
    1. K. L. Montgomery, A. J. Yeh, J. S. Ho, V. Tsao, S. Mohan Iyer, L. Grosenick, E. A. Ferenczi, Y. Tanabe, K. Deisseroth, S. L. Delp, A. S. Y. Poon, Nat. Methods 2015, 12, 969.
    1. A. D. Mickle, S. M. Won, K. N. Noh, J. Yoon, K. W. Meacham, Y. Xue, L. A. McIlvried, B. A. Copits, V. K. Samineni, K. E. Crawford, D. H. Kim, P. Srivastava, B. H. Kim, S. Min, Y. Shiuan, Y. Yun, M. A. Payne, J. Zhang, H. Jang, Y. Li, H. H. Lai, Y. Huang, S.-I. Park, R. W. Gereau, J. A. Rogers, Nature 2019, 565, 361.
    1. M. T. Alt, E. Fiedler, L. Rudmann, J. S. Ordonez, P. Ruther, T. Stieglitz, Proc. IEEE 2017, 105, 101.

LinkOut - more resources