Simplified turbidimetric determination of apolipoproteins A-I, A-II and B using a microtitre method

Abstract

A turbidimetric method is described for the determination of apolipoproteins A-I, A-II and B on microtitre plates. Regression analysis of the resulting values showed a good correlation to apolipoprotein values determined turbidimetrically on Cobas Bio (apolipoprotein A-I, A-II), and those determined by means of radial immuno diffusion (RID) (apolipoprotein A-I: r = 0.93, y = 1.02 x - 5.0, n = 63; apolipoprotein A-II: r = 0.90, y = 1.07 x - 5.6, n = 44; apolipoprotein B: r = 0.92, y = 0.95 x + 9.0, n = 58). The variation coefficient in the series was 3.5% (apolipoprotein A-I, n = 21), 2.5% (apolipoprotein A-II, n = 20) and 3.6% (apolipoprotein B, n = 19); and the variation coefficient from day to day 3.1% (apolipoprotein A-I, n = 45), 4.2% (apolipoprotein A-II, n = 39) and 5.3% (apolipoprotein B (n = 48).