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. 2019 Nov;152(5):345-353.
doi: 10.1007/s00418-019-01811-6. Epub 2019 Sep 5.

Metalloproteinase 14 (MMP-14) and hsa-miR-410-3p expression in human inflamed dental pulp and odontoblasts

Aniela Brodzikowska  1 , Agata Gondek  2 , Beata Rak  2   3   4 , Wiktor Paskal  2 , Kacper Pełka  2 , Agnieszka Cudnoch-Jędrzejewska  5 , Paweł Włodarski  2
Affiliations

Metalloproteinase 14 (MMP-14) and hsa-miR-410-3p expression in human inflamed dental pulp and odontoblasts

Aniela Brodzikowska et al. Histochem Cell Biol. 2019 Nov.

Abstract

The objective of this study is to evaluate MMP-14 expression in odontoblasts and in the bulk of dental pulp of teeth with pulpitis; to determine the expression of microRNA-410 (miR-410) in pulp tissue, since sequence analysis suggests that miR-410 has potential binding site on MMP-14's 3'UTR, and hence, can regulate expression of the latter one. Tissue samples of dental pulp from teeth with pulpitis and healthy (control) were formalin fixed and paraffin embedded (FFPE). Samples were examined using immunohistochemical staining for MMP-14 and the expression of miR-410 was evaluated using qRT-PCR. In both, healthy and inflamed pulp odontoblasts stained more intensively than remaining pulp tissue, but this difference was not statistically significant. More positive staining was observed in inflamed pulps compared to healthy pulps. Expression of miR-410 was found significantly lower in inflamed pulps than in healthy ones. In the two examined zones, odontoblasts and remaining pulp, miR-410 was expressed on a similar level. No statistically significant correlation of miR-410 and MMP-14 expression was found. We showed that inflammation changes the MMP-14 expression in pulp tissue and odontoblasts. This study demonstrates for the first time miR-410 expression in human dental pulp and that expression of this microRNA was downregulated in inflamed dental pulp and odontoblasts.

Keywords: MMP-14; Odontoblasts; Tooth pulp; microRNA-410.

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Conflict of interest statement

All authors declare that they have no conflict of interests.

Figures

Fig. 1
Fig. 1
Healthy (a–d) and inflamed (e–h) dental pulp. Each specimen was obtained from distinct patient. Immunohistochemical staining for MMP-14. Scale bar = 5 µm
Fig. 2
Fig. 2
The quantitative analysis of MMP-14 expression determined by IHC staining intensity in healthy and in inflamed pulp tissue by IHC Profiler in ImageJ (ImageJ v 1.48)
Fig. 3
Fig. 3
miR-410 expression level in healthy and inflamed pulp tissue. The expression of miR-410 was determined by qRT-PCR with U6 as an endogenous control and calculated by the comparative CT method
Fig. 4
Fig. 4
The relative level of the miR-410 expression over the endogenous control U6. The graph shows distinction into different subgroups: healthy, inflamed, pulp and odontoblasts. Median value is signed with line. The total level of the miR-410 expression in inflamed tissue was lower compared to healthy ones
See this image and copyright information in PMC

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