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. 2020 Jan;225(2):948-959.
doi: 10.1111/nph.16169. Epub 2019 Oct 19.

Wheat gene Sr60 encodes a protein with two putative kinase domains that confers resistance to stem rust

Shisheng Chen  1   2 , Matthew N Rouse  3 , Wenjun Zhang  1 , Xiaoqin Zhang  1 , Yan Guo  1 , Jordan Briggs  3 , Jorge Dubcovsky  1   4
Affiliations
Free article

Wheat gene Sr60 encodes a protein with two putative kinase domains that confers resistance to stem rust

Shisheng Chen et al. New Phytol. 2020 Jan.
Free article

Abstract

Wheat stem rust, caused by Puccinia graminis Pers. f. sp. tritici (Pgt), is a devastating fungal disease threatening global wheat production. The present paper reports the identification of stem rust resistance gene Sr60, a race-specific gene from diploid wheat Triticum monococcum L. that encodes a protein with two putative kinase domains. This gene, designated as WHEAT TANDEM KINASE 2 (WTK2), confers intermediate levels of resistance to Pgt. WTK2 was identified by map-based cloning and validated by transformation of a c.10-kb genomic sequence including WTK2 into susceptible common wheat variety Fielder (Triticum aestivum L.). Transformation of Fielder with WTK2 was sufficient to confer Pgt resistance. Sr60 transcripts were transiently upregulated 1 d post-inoculation with Pgt, but not in mock-inoculated plants. The upregulation of Sr60 was associated with stable upregulation of several pathogenesis-related genes. The Sr60-resistant haplotype found in T. monococcum was not found in polyploid wheat, suggesting an opportunity to introduce a novel resistance gene. Sr60 was successfully introgressed into hexaploid wheat, and we developed a diagnostic molecular marker to accelerate its deployment and pyramiding with other resistance genes. The cloned Sr60 also can be a useful component of transgenic cassettes including other resistance genes with complementary resistance profiles.

Keywords: Sr60; Triticum monococcum; kinase proteins; resistance genes; stem rust; wheat.

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