Nucleoporin 107 Promotes the Survival of Tumor Cells in Cervical Cancers

Abstract

Aims: Recent studies indicate that in addition to the construction of nuclear pore complex, nucleoporin (NUP)107 is actively involved in the pathogenesis of numerous cancer types, but the role of NUP107 in cervical carcinoma cells remains unknown.

Methods: We examined the expression of NUP107 in 30 cases of cervical carcinoma using quantitative reverse transcription-polymerase chain reaction and immunoblots. NUP107 stably overexpressing cell line was established to examine the function of NUP107 in cell viability, TUNEL assay, wound healing assay, 5-ethynyl-2'-deoxyuridine incorporation, and oxidative stress.

Results: NUP107 expression was significantly increased in the cervical carcinoma tissues, compared with their corresponding adjacent normal tissues. Overexpression of NUP107 conferred the cervical cancer cells with significant resistance to oxidative insult, but it had no effects on the migration and proliferation. This pro-survival function of NUP107 was associated with the elevated expression of Bcl-2, the activation of Akt signaling, and increased expression of nucleocytoplasmic transport factors. Silencing of NUP107 increased the sensitivity of cervical cancer cells to oxidative challenge, thereby inducing the apoptosis of cervical cancer cells.

Conclusion: NUP107 is significantly increased in cervical tissues and confers the cervical cancer cells with resistance to oxidative damage. These results provide an important role for specific NUP in mediating cervical cancer.

Keywords: Cervical cancer; Nucleoporin; Pro-survival.