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. 2019 Sep 4;24(18):3220.
doi: 10.3390/molecules24183220.

Quality Evaluation of Ophiopogonis Radix from Two Different Producing Areas

Mengxia Tan  1 , Jiali Chen  2 , Chengcheng Wang  3 , Lisi Zou  4 , Shuyu Chen  5 , Jingjing Shi  6 , Yuqi Mei  7 , Lifang Wei  8 , Xunhong Liu  9
Affiliations

Quality Evaluation of Ophiopogonis Radix from Two Different Producing Areas

Mengxia Tan et al. Molecules. .

Abstract

Ophiopogonis Radix, also known as Mai-dong in Chinese, was a commonly used traditional Chinese medicine (TCM) and functional health food. Two products of Ophiopogonis Radix are largely produced in the Sichuan and Zhejiang province, which are called "Chuan maidong (CMD)" and "Zhe maidong (ZMD)" respectively. To distinguish and evaluate the quality of CMD and ZMD, an analytical method based on ultra-fast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) was established for simultaneous determination of 32 constituents including 4 steroidal saponins, 3 homisoflavonoids, 15 amino acids, and 10 nucleosides in 27 Mai-dong samples from Sichuan and Zhejiang. Furthermore, principal components analysis (PCA), partial least squares discriminant analysis (PLS-DA), t-test, and grey relational analysis (GRA) were applied to discriminate and evaluate the samples from Sichuan and Zhejiang based on the contents of 32 constituents. The results demonstrated that the bioactive constituents in CMD and ZMD were significantly different, and CMD performed better in the quality assessment than ZMD. This study not only provides a basic information for differentiating CMD and ZMD, but offers a new insight into comprehensive evaluation and quality control of Ophiopogonis Radix from two different producing areas.

Keywords: Ophiopogonis Radix; different producing areas; multiple bioactive constituents; multivariate statistical analysis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Representative extract ions chromatograms (XIC) of multi-reaction monitoring (MRM) chromatograms of 32 investigated compounds. (Compounds were shown in Table 1).
Figure 2
Figure 2
The contents of four kinds of chemical components in Ophiopogonis Radix from different producing areas.
Figure 3
Figure 3
The PCA chromatogram of Chuan maidong (CMD) (A) and Zhe maidong (ZMD) (B).
Figure 4
Figure 4
The partial least squares discriminant analysis (PLS-DA) score scatter plot (a) and variable importance in the projection (VIP) (b) of Chuan maidong (CMD) (A) and Zhe maidong (ZMD) (B).
Figure 5
Figure 5
The box plot of 32 compounds contents in Chuan maidong (CMD) and Zhe maidong (ZMD) (*** p < 0.001).
Figure 5
Figure 5
The box plot of 32 compounds contents in Chuan maidong (CMD) and Zhe maidong (ZMD) (*** p < 0.001).
See this image and copyright information in PMC

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