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Comment
. 2019 Oct 1;116(40):19795-19796.
doi: 10.1073/pnas.1904767116. Epub 2019 Sep 5.

Inside-out signaling through FAK-integrin axis may regulate circulating cancer cell metastatic adhesion

Christina Downey-Biechler  1 , David H Craig  2 , Shyam K More  2 , Marc D Basson  3
Affiliations
Comment

Inside-out signaling through FAK-integrin axis may regulate circulating cancer cell metastatic adhesion

Christina Downey-Biechler et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

Conflict of interest statement: M.D.B. is coinventor of a pending patent application on the use of small molecules to inhibit FAK–Akt interaction to prevent cancer metastasis that has been jointly filed by the University of North Dakota and Michigan State University. No data from that patent application are presented here, however, and the FAK and Akt inhibitors used here are commercially available and not covered in that patent application. The authors have no other potential conflict of interest.

Figures

Fig. 1.
Fig. 1.
Force-activated signaling in metastasis. (A) Flow cytometry for 9EG7, FAKY397, and AktS473 in CSFE-stained CT-26 cells from cardiac puncture 30 min after BALB/c tail vein injection vs. uninjected controls (n = 6; *P < 0.05). (B) After preincubation with or without 15-mmHg increased pressure, with or without 20 µM FAK inhibitor Y15 or 1 µM Akt inhibitor IV, 750,000 CT-26-Luciferase cells were injected via BALB/c spleens. Typical pseudocolored bioluminescence (pink–red, low–high) at day 7. (C) Quantitation for B (n = 16; **P < 0.01 vs. nontreated control). (D) Splenic and hepatic weights (n = 16; *P < 0.05). (E) Survival of mice treated as in B [n = 20; P < 0.05 pressure vs. control, FAK inhibitor (P) or Akt inhibitor (P)]. (F) Typical pseudocolored images 7 d after 107 luciferase-expressing CT-26 cells pretreated with or without 15-mmHg pressure, with or without Y15 or Akt inhibitor, were injected into BALB/c mouse tail veins. (G) Quantitation for F (n = 20; *P < 0.05). (H) Survival as in F (n = 20 control–pressure, n = 12 for inhibitors; P < 0.05 control or pressure vs. each with FAK or Akt inhibitors).
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Comment on

References

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