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. 1988 Sep;20(2):167-70.
doi: 10.1016/0147-619x(88)90022-4.

Use of lacZ expression to monitor transcription

C S Tomich  1 , P S Kaytes, M K Olsen, H Patel
Affiliations

Use of lacZ expression to monitor transcription

C S Tomich et al. Plasmid. 1988 Sep.

Abstract

For use in monitoring transcription in operon fusion, we have constructed a lacZ sequence with the initiation codon ATG and the Escherichia coli consensus Shine-Dalgarno site. There are unique restriction endonuclease sites flanking the sequence to allow easy isolation of the lacZ sequence with or without the Shine-Dalgarno site. We have placed this lacZ sequence behind the bovine growth hormone (BGH) gene and found that the lacZ product beta-galactosidase synthesized reflects the level of BGH-specific mRNA.

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