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. 2020 Mar;65(2):465-470.
doi: 10.1111/1556-4029.14185. Epub 2019 Sep 6.

Age Estimation Based on DNA Methylation Using Blood Samples From Deceased Individuals

Helena Correia Dias  1   2   3 , Cristina Cordeiro  3   4 , Francisco Corte Real  3   4 , Eugénia Cunha  2   3 , Licínio Manco  1
Affiliations

Age Estimation Based on DNA Methylation Using Blood Samples From Deceased Individuals

Helena Correia Dias et al. J Forensic Sci. 2020 Mar.

Abstract

Age estimation using DNA methylation levels has been widely investigated in recent years because of its potential application in forensic genetics. The main aim of this study was to develop an age predictor model (APM) for blood samples of deceased individuals based in five age-correlated genes. Fifty-one samples were analyzed through the bisulfite polymerase chain reaction (PCR) sequencing method for DNA methylation evaluation in genes ELOVL2, FHL2, EDARADD, PDE4C, and C1orf132. Linear regression was used to analyze relationships between methylation levels and age. The model using the highest age-correlated CpG from each locus revealed a correlation coefficient of 0.888, explaining 76.3% of age variation, with a mean absolute deviation from the chronological age (MAD) of 6.08 years. The model was validated in an independent test set of 19 samples producing a MAD of 8.84 years. The developed APM seems to be informative and could have potential application in forensic analysis.

Keywords: DNA methylation age; bisulfite PCR sequencing; deceased individuals; forensic epigenetics; forensic science.

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