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Development of a qPCR-based whole blood prognostic biomarker. (A) Schematic depicting the workflow…
Figure 1
Development of a qPCR-based whole blood prognostic biomarker. (A) Schematic depicting the workflow for the development, optimisation and validation of the whole blood qPCR-based classifier with separate training and validation cohorts. (B) Distribution of correlation coefficients between microarray and qPCR-based measurements of gene expression for 39 genes. (C) Confidence of assignments to IBD1 and IBD2 subgroups in the training cohort using the qPCR classifier (15 informative and 2 reference genes). Colours indicate actual IBD1/IBD2 assignments based on CD8 T cell transcriptomic analysis (red=IBD1, blue=IBD2). Inset summary table depicts results using 0.5 cut-off for group assignment. CD, Crohn’s disease.
Figure 2
Validation of qPCR-based classifier in…
Figure 2
Validation of qPCR-based classifier in independent cohorts. (A and B) Kaplan-Meier plots of…
Figure 2
Validation of qPCR-based classifier in independent cohorts. (A and B) Kaplan-Meier plots of escalation-free survival for the CD validation cohort (A; n=66) and the UC validation cohort (B; n=57) as stratified by the IBDhi (IBD1 equivalent) and IBDlo (IBD2 equivalent) patient subgroups. Data are censored at 18 months. Statistical significance assessed by log-rank test. (C and D) Stacked density plots demonstrating the maximum medical therapy that was required during the first 2.5 years’ prospective follow-up of the IBDhi and IBDlo subgroups in CD (C) and UC (D). Treatments were plotted hierarchically (no treatment
Figure 3
The clinical course of Crohn’s…
Figure 3
The clinical course of Crohn’s disease (CD) is different in IBD1 and IBD2…
Figure 3
The clinical course of Crohn’s disease (CD) is different in IBD1 and IBD2 patients. (A) Kaplan-Meier plot of escalation-free survival for CD patients in the IBD1 and IBD2 subgroups. Data are censored at 18 months. Statistical significance assessed by log-rank test. (B and C) Kaplan-Meier plots in the same format as figure part A with patients subdivided according to clinical risk (high risk=2 or more of: age <40 years at diagnosis, early need for steroids and perianal disease; B) and presence of severe features at index endoscopy (deep and extensive ulceration in at least one colonic segment or endoscopist’s global assessment; C). (D) Stacked density plots demonstrating the maximum medical therapy that was required during 5 years’ prospective follow-up in the IBD1 and IBD2 subgroups. Treatments were plotted hierarchically (no treatment
Figure 4
The clinical course of UC…
Figure 4
The clinical course of UC is different in IBD1 and IBD2 patients. (A)…
Figure 4
The clinical course of UC is different in IBD1 and IBD2 patients. (A) Kaplan-Meier plot of escalation-free survival for UC patients in the IBD1 and IBD2 subgroups. Data are censored at 18 months. Statistical significance assessed by log-rank test. (B) Kaplan-Meier plot in the same format as figure part A with patients subdivided according to endoscopic disease severity at index colonoscopy. P value calculated by comparing mild and severe cases. (C) Stacked density plots demonstrating the maximum medical therapy that was required during the first 5 years’ prospective followup in the IBD1 and IBD2 subgroups. Treatments were plotted hierarchically (5-ASA only
Biasci D, Lee JC, Noor NM, Pombal DR, Hou M, Lewis N, Ahmad T, Hart A, Parkes M, McKinney EF, Lyons PA, Smith KGC.Biasci D, et al.Gut. 2019 Aug;68(8):1386-1395. doi: 10.1136/gutjnl-2019-318343. Epub 2019 Apr 27.Gut. 2019.PMID: 31030191Free PMC article.
