Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2019 Nov;38(11):2133-2143.
doi: 10.1007/s10096-019-03654-4. Epub 2019 Sep 7.

Comparison of rapid BACpro® II, Sepsityper® kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper® module analysis

Munevver Kayin  1 , Berivan Mert  2 , Söhret Aydemir  1 , Volkan Özenci  3   4
Affiliations
Comparative Study

Comparison of rapid BACpro® II, Sepsityper® kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper® module analysis

Munevver Kayin et al. Eur J Clin Microbiol Infect Dis. 2019 Nov.

Abstract

There are several approaches available for purifying microorganisms prior to matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. In the present study, rapid BACpro® II (Nittobo Medical Co., Ltd., Tokyo, Japan), a new application, has been compared with Sepsityper® kit (Bruker Daltonics, Billerica, USA) and an in-house method. Samples were also tested with two modules, standard and Sepsityper®, identified in the Bruker MALDI-TOF MS. The bottles having monomicrobial growth were included in the study according to Gram staining results. In total, two hundred blood culture bottles were included but there was no growth in one of the subcultures so 199 blood culture bottles were studied prospectively. With the standard MALDI-TOF MS analysis, rapid BACpro® II could successfully identify microorganisms in 174/199 (87.4%) of the bottles where Sepsityper® kit and in-house method were successful in 136/199 (68.3%) and 114/199 (57.3%), respectively. When the MALDI-TOF MS data were analysed by Sepsityper® module, the identification rates were increased to 94.4%, 82.1% and 69.8% (p < 0.001), respectively. In the Sepsityper® module, 72/73 (98.6%) of Gram-negative and 97/106 (91.5%) of Gram-positive microorganisms were detected by rapid BACpro® II method. The present study shows that rapid BACpro® II is a reliable preparation procedure and has higher rates of identification compared with Sepsityper® kit and in-house method. The use of the Sepsityper® module in blood cultures increases the chance of identification for all three methods studied.

Keywords: Blood culture; MALDI-TOF MS; Rapid BACpro II; Sepsityper® kit; Sepsityper® module.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Sample preparation steps for the three methods included in the study
Fig. 2
Fig. 2
Sample preparation with three different methods for identification of bacteria directly from blood culture bottles by MALDI-TOF MS analysed by standard module
Fig. 3
Fig. 3
Sample preparation with three different methods for identification of bacteria directly from blood culture bottles by MALDI-TOF MS analysed by Sepsityper® module
See this image and copyright information in PMC

References

    1. Paolucci M, Landini MP, Sambri V. Conventional and molecular techniques for the early diagnosis of bacteraemia. Int J Antimicrob Agents. 2010;36(Suppl 2):S6–S16. - PubMed
    1. Peralta G, Roiz MP, Sánchez MB, Garrido JC, Ceballos B, Rodríguez-Lera MJ, Mateos F, De Benito I. Time-to-positivity in patients with Escherichia coli bacteraemia. Clin Microbiol Infect. 2007;13:1077–1082. - PubMed
    1. Cattoir L, Coorevits L, Leroux-Roels I, Claeys G, Verhasselt B, Boelens J. Improving timelines in reporting results from positive blood cultures: simulation of impact of rapid identification on therapy on a real-life cohort. Eur J Clin Microbiol Infect Dis. 2018;37(12):2253–2260. - PubMed
    1. Seifert H. The clinical importance of microbiological findings in the diagnosis and management of bloodstream infections. Clin Infect Dis. 2009;48(Suppl 4):S238–S245. - PubMed
    1. Peters RP, van Agtmael MA, Danner SA, Savelkoul PH. Vandenbroucke-Grauls CM (2004) New developments in the diagnosis of bloodstream infections. Lancet Infect Dis. 2004;4(12):751–760. - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources