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. 2020 Mar;287(5):978-990.
doi: 10.1111/febs.15058. Epub 2019 Sep 18.

Long non-coding RNA RP11-820 promotes extracellular matrix production via regulating miR-3178/MYOD1 in human trabecular meshwork cells

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Long non-coding RNA RP11-820 promotes extracellular matrix production via regulating miR-3178/MYOD1 in human trabecular meshwork cells

Wencui Shen et al. FEBS J. 2020 Mar.
Free article

Abstract

Primary open angle glaucoma (POAG) is the most common type of glaucoma. At the mechanistic level, POAG is thought to be caused by extracellular matrix (ECM) deposition in the trabecular meshwork (TM). Growing evidence has shown that long noncoding RNAs (lncRNAs) are involved in the fibrotic process underlying many diseases. This study was undertaken to explore the role of lncRNA-RP11-820 in ECM production of human TM cells (HTMCs). Our results revealed that lncRNA-RP11-820 was significantly upregulated under oxidative stress in HTMCs. Further investigation revealed that lncRNA-RP11-820 directly binds miR-3178, through which the expression of MYOD1 is regulated. Importantly, MYOD1 can transcriptionally activate ECM genes in HTMCs, in complex with STAT3. Taken together, our data established that oxidative stress-induced lncRNA-RP11-820 plays a key role in regulating the miR-3178/MYOD1/ECM axis in HTMCs. These findings further elucidate the pathogenic mechanism and provide a novel therapeutic target relevant to POAG.

Keywords: extracellular matrix; gene regulation; long noncoding RNA; primary open angle glaucoma.

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References

    1. Jonas JB, Aung T, Bourne RR, Bron AM, Ritch R & Panda-Jonas S (2017) Glaucoma. Lancet 390, 2183.
    1. Fingert JH (2011) Primary open-angle glaucoma genes. Eye 25, 587-595.
    1. Liu Y & Allingham RR (2017) Major review: molecular genetics of primary open-angle glaucoma. Exp Eye Res 160, 62-84.
    1. Anderson DR (2011) Normal-tension glaucoma (Low-tension glaucoma). Indian J Ophthalmol 59, S97-S101.
    1. Masihzadeh O, Lei TC, Ammar DA, Kahook MY & Gibson EA (2012) A multiphoton microscope platform for imaging the mouse eye. Mol Vis 18, 1840-1848.

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