Formation of the tooth enamel rod pattern and the cytoskeletal organization in secretory ameloblasts of the rat incisor

Abstract

The localization of actin, myosin, tropomyosin, alpha-actinin, vinculin, and desmoplakin I/II was visualized by immunofluorescence microscopy. Antibodies against myosin, tropomyosin, and alpha-actinin and rhodamine-phalloidin labeled strongly the proximal and distal terminal webs which ultrastructurally consist of dense microfilament bundles. In the distal terminal web, the staining by these reagents occurred mostly perpendicular to the long axis of the incisor. Antivinculin stained the general area where the distal terminal web is located in the ameloblast. Anti-desmoplakin I/II labeled the junctional area associated with the proximal and distal terminal webs. The anti-desmoplakin staining was stronger along the cell border perpendicular to the long axis of the incisor. Comparison of the rhodamine-phalloidin staining pattern of the distal terminal web and the enamel secretion pattern by ameloblasts revealed that a change in the distal terminal web staining pattern preceded a change in the secretion pattern. These observations suggest that the cytoskeletal organization in the ameloblast is involved in the formation of the enamel matrix pattern in the rat incisor.