Nanotechnology in Dentin Disinfection: Can We Preserve the Bond?

Abstract

Aims: The aim of this study is to evaluate the effect of cavity disinfection with 2% chlorhexidine (CHX) and Ag-Au nanoparticles on microleakage and resin tag penetrability of composite restoration under in vitro conditions.

Materials and methods: Twenty-five human permanent molars extracted for therapeutic reasons were used in the study. Class V cavity of standard dimension was prepared on the buccal and lingual surfaces of the teeth. The teeth were randomly allocated into two groups based on the cavity disinfectant used: group I being 2% CHX gluconate (chlorhexidina Friedrich and Bianca Mittelstadt (FGM)) and group II being cavity disinfectant containing Ag-Au nanoparticles (nanocare gold). In both the groups, the dentin was etched with 37% phosphoric acid and cavity disinfectants were applied following which dentine bonding agent and composite resin were applied and cured. The specimens were then viewed under a stereomicroscope and a scanning electron microscope for microleakage and resin tag formation, respectively.

Results: The results were statistically analyzed using an independent "t" test. No significant difference was seen between the two groups with respect to both microleakage and resin tag penetration values (p > 0.05).

Conclusion: The cavity disinfectant containing Ag-Au nanoparticles did not affect the sealing ability and resin tag penetrability of composite resin in permanent molars when compared with 2% CHX.

How to cite this article: Attiguppe P, Tripathi AP, et al. Nanotechnology in Dentin Disinfection: Can We Preserve the Bond? Int J Clin Pediatr Dent 2019;12(1):42-46.

Keywords: Ag–Au (silver–gold) nanoparticles; Chlorhexidine; Microleakage; Resin tag.

Fig. 1

Representative sample from Group II after FEI Scanning electron microscope evaluation showing resin tags in μm at 5000× mag

Fig. 2

Mean comparison of microleakage observed using cavity disinfectants containing CHX and Ag–Au nanoparticles at gingival margin

Fig. 3

Mean comparison of microleakage observed using cavity disinfectants containing CHX and Ag–Au nanoparticles at occlusal margin

Fig. 4

Mean comparison of resin tags observed using cavity disinfectants containing CHX and Ag–Au nanoparticles

Fig. 5

Representative sample from Group I after FEI scanning electron microscope evaluation showing length of the resin tag ranging from 9.75 μm to 18.90 μm (1000× mag)

Fig. 6

Representative sample from Group II after FEI Scanning electron microscope evaluation showing length of the resin tag ranging from 7.010 μm to 15.89 μm (2000× mag)