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Review
. 2019 Aug 21:7:201.
doi: 10.3389/fbioe.2019.00201. eCollection 2019.

Omics Application of Bio-Hydrogen Production Through Green Alga Chlamydomonas reinhardtii

Lili Xu  1 , Jianhua Fan  2   3 , Quanxi Wang  1
Affiliations
Review

Omics Application of Bio-Hydrogen Production Through Green Alga Chlamydomonas reinhardtii

Lili Xu et al. Front Bioeng Biotechnol. .

Abstract

This article summarizes the current knowledge regarding omics approaches, which include genomics, transcriptomics, proteomics and metabolomics, in the context of bio-hydrogen production in Chlamydomonas reinhardtii. In this paper, critical genes (HydA1, Hyd A2, Sulp, Tla1, Sta7, PFL1) involved in H2 metabolism were identified and analyzed for their function in H2 accumulation. Furthermore, the advantages of gene microarrays and RNA-seq were compared, as well as their applications in transcriptomic analysis of H2 production. Moreover, as a useful tool, proteomic analysis could identify different proteins that participate in H2 metabolism. This review provides fundamental theory and an experimental basis for H2 production, and further research effort is needed in this field.

Keywords: Chlamydomonas reinhardtii; genomics; hydrogen production; metabolomics; proteomics; transcriptomics.

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Figures

Figure 1
Figure 1
Model diagram of hydrogen production of C. reinhardtii.
Figure 2
Figure 2
Aerobic (A) and anaerobic (B) stages of two phases H2 production and Micro-oxic continuous H2 production (C). Proton(H+) flow is marked with dashed lines, electron flow with continuous lines.
See this image and copyright information in PMC

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