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Comment
. 2019 Dec 1;74(12):3626-3628.
doi: 10.1093/jac/dkz363.

Detection of a novel mcr-5.4 gene variant in hospital tap water by shotgun metagenomic sequencing

Giuseppe Fleres  1 , Natacha Couto  1 , Leonard Schuele  1 , Monika A Chlebowicz  1 , Catarina I Mendes  1 , Luc W M van der Sluis  2 , John W A Rossen  1 , Alex W Friedrich  1 , Silvia García-Cobos  1
Affiliations
Comment

Detection of a novel mcr-5.4 gene variant in hospital tap water by shotgun metagenomic sequencing

Giuseppe Fleres et al. J Antimicrob Chemother. .
No abstract available

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Figures

Figure 1
Figure 1
Comparative analysis of the genetic environment of mcr-5 between the reference plasmid pSE13-SA01718 (accession no. KY807921.1) and the annotated hybrid metagenome contig (accession no. MK965519). The contig carrying the mcr-5.4 gene consists of the following putative gene products: 7-carboxy-7-deazaguanine synthase (queE), 7-cyano-7-deazaguanine synthase (queC), glycine cleavage system transcriptional antiactivator GcvR (gcvR), thiol peroxidase (tpx), sulphurtransferase TusA family protein (sirA), hypothetical protein (hp), truncated MFS-type transporter (Δmsf), lipid A phosphoethanolamine transferase (mcr-5.4), ChrB domain protein (chrB), transposon resolvase (tnpR) and truncated transposon transposase (ΔtnpA). Areas with 98% identity between sequences are represented in light grey. Arrows indicate the position and direction of the genes. The transposon Tn6452 sequence in the reference plasmid pSE13-SA01718 is bounded by inverted repeats: IRL and IRR.
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References

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Supplementary concepts