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. 2019 Apr-Jun;15(2):187-194.
doi: 10.4183/aeb.2019.187.

GALLIC ACID IMPROVES OXIDATIVE STRESS AND INFLAMMATION THROUGH REGULATING MICRORNAS EXPRESSIONS IN THE BLOOD OF DIABETIC RATS

F Ramezani Ali Akbari  1   2 , M Badavi  1   2   3 , M Dianat  1   2 , S A Mard  1   2 , A Ahangarpour  1   2   4
Affiliations

GALLIC ACID IMPROVES OXIDATIVE STRESS AND INFLAMMATION THROUGH REGULATING MICRORNAS EXPRESSIONS IN THE BLOOD OF DIABETIC RATS

F Ramezani Ali Akbari et al. Acta Endocrinol (Buchar). 2019 Apr-Jun.

Abstract

Context: Endothelial dysfunction and diabetic cardiomyopathy are critical complications of diabetes. Gallic acid (GA) plays a significant role in cardiovascular disorders resulted from diabetes. In addition, increased plasma miR-24, miR-126 associated with endothelial dysfunction.

Aim: The current study was designed to assess the effects of GA on plasma miR-24, miR-126 levels in the diabetic rats.

Animals and methods: Adult male Sprague-Dawley rats were divided into three groups (n=8): control (C), diabetic (D) and diabetic group treated with GA (D+G, 25 mg/kg, by gavage) for eight weeks. The blood glucose level, body weight, lipid profile, blood pressure, plasma miR-24 and miR-126 levels, antioxidant and inflammatory biomarkers were measured.

Results: The plasma levels of miR-24, miR-126, body weight, high-density lipoprotein cholesterol (HDL-c), total anti-oxidant capacity (TAC) and the systolic blood pressure significantly reduced and blood glucose, total cholesterol (TC), triglycerides (TG), very low-density lipoprotein cholesterol (VLDL-c), malondialdehyde (MDA), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and low-density lipoprotein cholesterol (LDL-c) significantly elevated among the diabetic rats compared with the control group. However, GA restored body weight, blood pressure, TC, TG, VLDL-c, TNF-α, miR-126, blood glucose, HDL-c, MDA, TAC, miR-24 and IL-6 among the GA treated rats compared with the diabetic group.

Conclusion: GA improves inflammation, oxidative stress and hypotension result from diabetes. These protective effects are probably mediated via increasing plasma miR-24 and miR-126 levels.

Keywords: Diabetes; Endothelium; Gallic acid; MicroRNA.

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Conflict of interest statement

The author declared no potential conflict of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1.
Figure 1.
Body weight (a) and blood glucose level (b) (mean ± SEM, n = 8) in control (C), diabetic (D) and diabetic treated with GA (25 mg/kg, D+G) before and after eight weeks of treatment.
Figure 2.
Figure 2.
Data were analyzed by one-way ANOVA followed by LSD post hoc test. ###p<0.001 versus control group, **p<0.01 versus untreated diabetic group.
Figure 3.
Figure 3.
Significance was analyzed by one-way ANOVA followed by LSD post hoc test. #p<0.05, ##p<0.01, ###p<0.001, versus control group. *p<0.05, ***p<0.001 versus untreated diabetic group.
Figure 4.
Figure 4.
Data were analyzed by one-way ANOVA followed by LSD post hoc test. #p<0.05, ##p<0.01, ###p<0.001, versus control group, **p<0.01, ***p<0.001 versus untreated diabetic group.
Figure 5.
Figure 5.
Data were analyzed by one-way ANOVA followed by LSD post hoc test. ###p<0.001 versus control group, *p<0.05 versus untreated diabetic group.
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