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. 2019 May;13(2):238-242.
doi: 10.1055/s-0039-1694697. Epub 2019 Sep 11.

Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells

Sri Wigati Mardi Mulyani  1 , Eha Renwi Astuti  1 , Otty Ratna Wahyuni  1 , Diah Savitri Ernawati  2 , Nastiti Faradilla Ramadhani  1
Affiliations

Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells

Sri Wigati Mardi Mulyani et al. Eur J Dent. 2019 May.

Abstract

Objectives: The aim of this study was to describe the process of regeneration of damaged salivary glands due to ionizing radiations by bone marrow mesenchymal stem cells (BM-MSCs) transplantation that have been given hypoxic preconditioning with 1% O2 concentration.

Materials and methods: Stem cell culture was performed under normoxic (O2: 21%) and hypoxic conditions by incubating the cells for 48 hours in a low oxygen tension chamber consisting of 95% N2, 5% CO2, and 1% O2. Thirty male Wistar rats were divided into four groups: two groups of control and two groups of treatment. A single dose of 15 Gy radiation was provided to the ventral region of the neck in all treatment groups, damaging the salivary glands. BM-MSCs transplantation was performed in the treatment groups for normoxia and hypoxia 24-hour postradiation.

Statistical analysis: Statistical analysis was done using normality test, followed by MANOVA test (p < 0.05).

Results: There was a significant difference in the expression of binding SDF1-CXCR4, Bcl-2 (p < 0.05) and also the activity of the enzyme α-amylase in all groups of hypoxia.

Conclusion: BM-MSCs transplantation with hypoxic precondition increases the expression of binding SDF1-CXCR4, Bcl-2 that contributes to cell migration, cell survival, and cell differentiation.

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Conflict of interest statement

None declared.

Figures

Fig. 1
Fig. 1
Comparison of CXCR4 (brown chromogen) expression between treatments. In the slide it appears that hypoxic group in acute conditions showed mainly CXCR4 expression ( A ) that was stronger than that in the normoxic group ( B ). Immunohistochemical staining, 400× magnification; Nikon H600L microscope; 300-megapixel camera DS Fi2.
Fig. 2
Fig. 2
Microscopic images of BM-MSCs labeled using PKH 26. The green light in the image shows the distribution of labeled BM-MSCs. Hypoxic condition ( A ) , normoxic condition ( B ).
Fig. 3
Fig. 3
Comparison of the expression of Bcl-2 (brown chromogen) between treatments. In the slide, it appears that groups with acute hypoxia ( A ) showed stronger Bcl-2 expression compared with those in the acute normoxic group ( B ) . Immunohistochemical staining, 400× magnification; Nikon H600L microscope; 300-megapixel camera DS Fi2.
See this image and copyright information in PMC

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