Bacteroides faecalis sp. nov., isolated from human faeces

Abstract

A novel Gram-stain-negative and strictly anaerobic bacterial strain, designated KGMB02408^T, was isolated from faeces of a healthy human in the Republic of Korea. The isolate was characterized as non-motile, non-spore-forming and rod-shaped (variable in length). The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KGMB02408^T belonged to the genus Bacteroides and was most closely related to Bacteroides faecichinchillae JCM 17102^T (=KCTC 15666^T; 96.5 %). Based on its whole-genome sequence, the DNA G+C content of the isolate was 39.5 mol%. The average nucleotide identity value between strain KGMB02408^T and related species, B. faecichinchillae JCM 17102^T, was 93.8 %. The major cellular fatty acids (>10 %) of the isolate were anteiso-C_{15 : 0}, iso-C_{17 : 0}-OH, summed feature 11 (iso-C_{17 : 0}-OH and/or C_{18 : 2} DMA) and C_{16 : 0}. Menaquinone-8 (28.6 %) and menaquinone-10 (47.1 %) were detected as the major respiratory quinones in the isolate. The major end products of glucose fermentation produced by strain KGMB02408^T were lactic acid, acetic acid and formic acid. Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain KGMB02408^T represents a novel species of the genus Bacteroides in the family Bacteroidaceae. The type strain is KGMB02408^T (=KCTC 15687^T=DSM 107828^T).

Keywords: Bacteroides faecalis; human faeces; microbiota; taxonomy.