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Comment
. 2020 Feb;72(2):374-376.
doi: 10.1002/art.41102. Epub 2019 Dec 27.

Reactivity of IgG With the p40 Protein Encoded by the Long Interspersed Nuclear Element 1 Retroelement: Comment on the Article by Carter et al

Mary K Crow  1
Affiliations
Comment

Reactivity of IgG With the p40 Protein Encoded by the Long Interspersed Nuclear Element 1 Retroelement: Comment on the Article by Carter et al

Mary K Crow. Arthritis Rheumatol. 2020 Feb.
No abstract available

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Figures

None
Reactivity of SLE and healthy donor IgG with LINE-1 p40 fusion protein.
An L1 ORF1 sequence subcloned from the embryonal carcinoma NTERA-2 clone D1 cell line was cloned into a pQE-30 expression vector. E.coli were transformed and p40 protein expressed and purified with a histidine tag. A. 700 ng p40 fusion protein per lane was electrophoresed and transferred to PVDF membranes, cut into strips, immunoblotted with 1/100 sera from SLE patients or healthy donors and developed with anti-human IgG. B. Microwells were coated with 500 ng/well of L1 p40 fusion protein, blocked with 5% milk, and incubated overnight with a 1/50 dilution of SLE or healthy donor sera. In addition, 5 samples from patients with other autoimmune rheumatic diseases (3 polymyositis, 1 Sjogren’s syndrome, 1 psoriatic arthritis) were assayed. After washing, alkaline phosphatase-labeled goat anti-human IgG was added and the color reaction measured at 405 nm absorbance. The presented experiments were performed by Jun Liang Zhou. This work was supported by NIH R21AR050673 and a Novel Research Grant from the Lupus Research Institute to MKC.
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  • Reply.
    Mustelin T, Ukadike K, LaCava J, Taylor M. Mustelin T, et al. Arthritis Rheumatol. 2020 Feb;72(2):376-377. doi: 10.1002/art.41104. Epub 2019 Dec 29. Arthritis Rheumatol. 2020. PMID: 31513363 No abstract available.

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References

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