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. 2019 Sep;26(6):1298-1304.
doi: 10.1016/j.sjbs.2019.04.014. Epub 2019 Apr 18.

Influence of gibberellic acid and different salt concentrations on germination percentage and physiological parameters of oat cultivars

Anju Chauhan  1 , Bassam A AbuAmarah  2 , Atul Kumar  1 , J S Verma  3 , Hamed A Ghramh  4   5   6 , Khalid Ali Khan  4   5   6 , Mohammad Javed Ansari  7
Affiliations

Influence of gibberellic acid and different salt concentrations on germination percentage and physiological parameters of oat cultivars

Anju Chauhan et al. Saudi J Biol Sci. 2019 Sep.

Abstract

Gibberellic acid (GA3) is one of the plant growth regulators which improve salt tolerance and mitigate the salt stress impact on plants. The extant analysis was carried out to study the effect of GA3 and different salt concentrations on seed germination and physiological parameters of oat cultivars. Oats is substantially less tolerant to salt than wheat and barley. Experimentation was conducted as factorial with Completely Randomized Block Design with three replicates. Different concentration of NaCl salt ((25, 50, 75 and 100 mM) were used in test control group and 100 and 150 ppm of GA3 were used in two group by pre-treated (after 24 h of the seed soaking) and plants were analyzed on 15th day. Results indicate that increasing salinity would decrease the germination percentage and growth parameter in three oat cultivars. Quotes data indicating a 13%, 19.9% and 32.48% in cultivars NDO-2, UPO-212 and UPO-94 germination reduction when soil salinity reaches 50 mM. A 36.02%, 47.33% and 56.365 reduction in germination is likely when soil salinity reaches 100 mM respectively same cultivars. Seeds treated with GA3 significantly promoted the percentage of germination, shoot and root length, total fresh and dry weight of seedling, tissue water content and seedling vigor index by NDO-2 and UPO-212 under different saline concentration. The maximum average of germination and growth parameters were observed from 150 ppm GA3 treated seeds. But this concentration was significantly inhibited root length in sensitive cultivar UPO-94 at 75 and 100 mM salt as compared to 100 ppm. We observed that, the high concentration of GA3 was not suitable for sensitive oat cultivars. Because the plant root are the real workforce behind any plants success. Thus, it may be concluding that, GA3 treatment could curtail the toxic effect of salinity by increasing germination percentage and shoot and root length, total fresh and dry weight, tissue water content and seedling vigor index in tolerant cultivar.

Keywords: Germination; Gibberellic acid; Oat cultivars; Salt concentrations; Seedling vigor index; Seedlings growth.

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Figures

Fig. 1
Fig. 1
Effects of NaCl and GA3 concentrations on (a) seed germination and (b) length of shoot and (c) length of root of three oat cultivars.
Fig. 2
Fig. 2
Combined effects of NaCl and GA3 concentrations on (a) TFW of seedling, (b) TDW of seedling and (c) TWC of seedling of three oat cultivars. Application of GA3 (150 ppm) significantly promoted TFW, TDW and TWC of seedling as compared to 100 ppm GA3 under different salt concentrations.
Fig. 3
Fig. 3
Combined effects of NaCl and GA3 concentrations on (a) seedling vigour ondex (I) and (b) seedling vigour ondex (II) of three oat cultivars. Application of GA3 (150 ppm) significantly promoted seedling vigour ondex (I & II) as compared to 100 ppm GA3 under different salt concentrations.
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