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. 2019:2032:203-211.
doi: 10.1007/978-1-4939-9650-6_12.

Immunophenotyping of Circulating Endothelial Cells and Endothelial Microparticles

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Immunophenotyping of Circulating Endothelial Cells and Endothelial Microparticles

Nicholas Wanner et al. Methods Mol Biol. 2019.

Abstract

Flow-cytometric detection of circulating endothelial cells and endothelial microparticles is an essential tool in studies of vascular diseases. Here we describe the principles and detailed methods for human blood sample processing, storage, labeling, and gating of circulating endothelial elements.

Keywords: Endothelial; Flow cytometry; Immunophenotyping; Microparticle.

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