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. 2019 Aug 28;15(4):512-517.
doi: 10.12965/jer.1938380.190. eCollection 2019 Aug.

Effects of a single bout of exercise on mitochondria-mediated apoptotic signaling in rat cardiac and skeletal muscles

Su-Zi Yoo  1 , Mi-Hyun No  1 , Jun-Won Heo  1 , Eunwook Chang  1 , Dong-Ho Park  1 , Ju-Hee Kang  2 , Dae-Yun Seo  3 , Jin Han  3 , Su-Jeen Jung  4 , Kwan Hwangbo  5 , Hyo-Bum Kwak  1
Affiliations

Effects of a single bout of exercise on mitochondria-mediated apoptotic signaling in rat cardiac and skeletal muscles

Su-Zi Yoo et al. J Exerc Rehabil. .

Abstract

This study aimed to determine the effects of a single bout exercise on mitochondria-mediated apoptotic signaling in cardiac and skeletal muscles. Fischer 344 rats (4 months old) were randomly divided into the control or a single bout of exercise group (n=10 each). The rats performed a single bout of treadmill exercise for 60 min. Mitochondria-mediated apoptotic signaling (e.g., Bax, Bcl-2, mitochondrial permeability transition pore [mPTP] opening, cytochrome c, and cleaved caspase-3) was measured in cardiac (e.g., left ventricle) and skeletal (e.g., soleus and white gastrocnemius) muscles. A single bout of exercise significantly decreased mPTP opening sensitivity in all tissues. However, a single bout of exercise did not show any statistical differences in Bax, Bcl-2, cytochrome c, and cleaved caspase-3 in all tissues measured. A single bout of exercise did not show definite results on characteristics of mitochondria-mediated apoptotic signaling. Therefore, further research is necessary to provide a more mechanistic understanding of the apoptosis pathway.

Keywords: Apoptosis; Exercise; Heart; Mitochondria; Skeletal muscle.

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Conflict of interest statement

CONFLICT OF INTEREST No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1
Effects of a single bout of exercise on Bax protein level in left ventricle (A), soleus (B), and white gastrocnemius (C). The data are shown as mean±standard error of the mean. CON, control group; EX, exercise group; AU, arbitrary unit; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Fig. 2
Fig. 2
Effects of a single bout of exercise on Bcl-2 protein level in left ventricle (A), soleus (B), and white gastrocnemius (C). The data are shown as mean±standard error of the mean. CON, control group; EX, exercise group; AU, arbitrary unit; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Fig. 3
Fig. 3
Effects of a single bout of exercise on Bax/Bcl-2 ratio in left ventricle (A), soleus (B), and white gastrocnemius (C). The data are shown as mean±standard error of the mean. CON, control group; EX, exercise group.
Fig. 4
Fig. 4
Effects of a single bout of exercise on mitochondrial permeability transition pore (PTP) opening sensitivity in left ventricle (A), soleus (B), and white gastrocnemius (C). The data are shown as mean±standard error of the mean. CON, control group; EX, exercise group; AU, arbitrary unit; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. *P<0.05 compared to the control group.
Fig. 5
Fig. 5
Effects of a single bout of exercise on cytochrome c protein level in left ventricle (A), soleus (B), and white gastrocnemius (C). The data are shown as mean± standard error of the mean. CON, control group; EX, exercise group; AU, arbitrary unit; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Fig. 6
Fig. 6
Effects of a single bout of exercise on cleaved caspase-3 protein level in left ventricle (A), soleus (B), and white gastrocnemius (C). The data are shown as mean±standard error of the mean. CON, control group; EX, exercise group; AU, arbitrary unit; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
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References

    1. Boroujerdi SS, Rahimi R. The apoptotic response to resistance exercise with different intensities in athletes. Med Sport. 2011;64:31–44.
    1. Gleeson M, Bishop NC, Stensel DJ, Lindley MR, Mastana SS, Nimmo MA. The anti-inflammatory effects of exercise: mechanisms and implications for the prevention and treatment of disease. Nat Rev Immunol. 2011;11:607–615. - PubMed
    1. Gram M, Vigelsø A, Yokota T, Helge JW, Dela F, Hey-Mogensen M. Skeletal muscle mitochondrial H2O2 emission increases with immobilization and decreases after aerobic training in young and older men. J Physiol. 2015;593:4011–4027. - PMC - PubMed
    1. Heo JW, No MH, Min DH, Kang JH, Kwak HB. Aging-induced sarcopenia and exercise. Asian J Kinesiol. 2017;19:43–59.
    1. Konopka AR, Suer MK, Wolff CA, Harber MP. Markers of human skeletal muscle mitochondrial biogenesis and quality control: effects of age and aerobic exercise training. J Gerontol A Biol Sci Med Sci. 2014;69:371–378. - PMC - PubMed