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. 2019 Oct;16(10):979-982.
doi: 10.1038/s41592-019-0553-1. Epub 2019 Sep 16.

Liquid application method for time-resolved analyses by serial synchrotron crystallography

Pedram Mehrabi #  1 , Eike C Schulz #  1   2 , Michael Agthe  3 , Sam Horrell  3 , Gleb Bourenkov  4 , David von Stetten  4 , Jan-Philipp Leimkohl  5 , Hendrik Schikora  5 , Thomas R Schneider  4 , Arwen R Pearson  2   3 , Friedjof Tellkamp  5 , R J Dwayne Miller  6   7   8
Affiliations

Liquid application method for time-resolved analyses by serial synchrotron crystallography

Pedram Mehrabi et al. Nat Methods. 2019 Oct.

Abstract

We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.

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