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. 2019 Jul;12(7):1101-1107.
doi: 10.14202/vetworld.2019.1101-1107. Epub 2019 Jul 25.

The concentration of testosterone, pituitary adenylate cyclase-activating polypeptide, and protamine 1 in the serum of male chicken following administration of epididymis and testicular extracts and their combination

Muslim Akmal  1 , Gholib Gholib  2 , Rinidar Rinidar  3 , Fitriani Fitriani  1 , T Zahrial Helmi  4 , Sugito Sugito  5 , M Isa  4 , Nurliana Nurliana  6 , Sri Wahyuni  7 , Dasrul Dasrul  8 , M Aman Yaman  9
Affiliations

The concentration of testosterone, pituitary adenylate cyclase-activating polypeptide, and protamine 1 in the serum of male chicken following administration of epididymis and testicular extracts and their combination

Muslim Akmal et al. Vet World. 2019 Jul.

Abstract

Bakcground and aim: Testis and epididymis are male reproductive organs that play an important role in spermatogenesis. These two organs are rich in the content of hormones and other molecules needed in the process of spermatogenesis which affect the quality of the spermatozoa. The objective of this study was to examine the effect of the administration of epididymis and testicular extracts and their combination on testosterone, pituitary adenylate cyclase-activating polypeptide (PACAP), and protamine 1 (PRM1) concentrations in the serum of male chicken.

Materials and methods: Twenty male chickens (broiler strain Cp707), aged 3 weeks and weighing 800-1000 g, were randomly divided into four different groups including a control group (T0) = injected with 1 ml normal saline and treatment groups: T1 = injected with 1 ml epididymis extract, T2 = injected with 1 ml testicular extract, and T3 = injected with a combination of 1 ml epididymis + 1 ml testicular extract. The experiment was conducted for 13 days and at the end of the study (day 14), the chickens were sacrificed to obtain the serum. Furthermore, the concentrations of testosterone, PACAP, and PRM1 were then measured by using an enzyme-linked immunosorbent assay technique.

Results: The concentrations of PACAP and PRM1 did not show a significant difference between treatment groups (T1, T2, and T3) and control group (T0) (p>0.05). However, the concentration of testosterone showed a significantly higher difference in a group injected with a combination of 1 ml epididymis and 1 ml testicular extracts (T3) compared to the control group (T0) (p<0.05).

Conclusion: The administration of epididymis and testicular extracts and their combination did not affect the increase of PACAP and PRM1 concentration. However, a combination of these extracts significantly affects the increase of testosterone concentration in the serum of male chicken.

Keywords: chicken; epididymis and testicular extracts; pituitary adenylate cyclase-activating polypeptide; protamine 1; spermatogenesis; testosterone.

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Figures

Figure-1
Figure-1
The concentration (mean ± standard deviation) of testosterone (a), pituitary adenylate cyclase-activating polypeptide (b), and protamine 1 (c) after injected with epididymis and testicular extracts and their combination in male chicken. Different superscripts indicate a statistically significant difference between groups (p<0.05).
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