Application of slightly acidic electrolyzed water and ultraviolet light for Salmonella enteritidis decontamination of cell suspensions and surfaces of artificially inoculated plastic poultry transport coops and other facility surfaces

Abstract

The efficiency of combination treatment of slightly acidic electrolyzed water (SAEW) and ultraviolet light (UV) for inactivation of Salmonella enteritidis (S. enteritidis) on the surface of plastic poultry coops and other facility surfaces was evaluated in the presence of organic matter. The bactericidal activities of SAEW, UV + SAEW, and composite phenol (CP) for inactivating S. enteritidis were also compared. Moreover, a model of UV + SAEW treatment of plastic transport coops with different times and available chlorine concentrations (ACC) was developed using multiple linear regression analysis. There are differences between SAEW and CP inactivation of S. enteritidis on coops, stainless steel, and glass surfaces (P < 0.05), and there are no differences between SAEW and CP on tire surfaces (P > 0.05). Disinfection of some rough material surfaces with SAEW treatment alone under feces interference on poultry farms may need a longer treatment time and/or a higher ACC than smooth surfaces. The combined treatment of UV and SAEW showed higher inactivation efficiency of S. enteritidis compared to CP and SAEW treatment alone (P < 0.05) in pure cultures or on the facility surfaces. A complete 100% inactivation of S. enteritidis on plastic poultry coop surfaces was obtained by using UV + SAEW with an ACC of 90 mg/L for more than 70 s. The established model had a good fit that was quantified by the determination coefficient R2 (0.93) and a lack of fit test (P > 0.05). The bactericidal efficiency of UV + SAEW increased with greater ACC and increasing time. The findings of this study indicate that the combination treatment of UV and SAEW may be a promising disinfection method and could be used instead of SAEW alone, especially on rough materials in the presence of organic matter on poultry farms.

Keywords: Salmonella enteritidis; facility surfaces; slightly acidic electrolyzed water; ultraviolet.