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. 2020 Jan;101(2):310-323.
doi: 10.1111/tpj.14542. Epub 2019 Oct 22.

AtPER1 enhances primary seed dormancy and reduces seed germination by suppressing the ABA catabolism and GA biosynthesis in Arabidopsis seeds

Huhui Chen  1 , Jiuxiao Ruan  1 , Pu Chu  2 , Wei Fu  1 , Zhenwei Liang  1 , Yin Li  1 , Jianhua Tong  3 , Langtao Xiao  3 , Jun Liu  4 , Chenlong Li  1 , Shangzhi Huang  1
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Free article

AtPER1 enhances primary seed dormancy and reduces seed germination by suppressing the ABA catabolism and GA biosynthesis in Arabidopsis seeds

Huhui Chen et al. Plant J. 2020 Jan.
Free article

Abstract

Seed is vital to the conservation of germplasm and plant biodiversity. Seed dormancy is an adaptive trait in numerous seed-plant species, enabling plants to survive under stressful conditions. Seed dormancy is mainly controlled by abscisic acid (ABA) and gibberellin (GA) and can be classified as primary and secondary seed dormancy. The primary seed dormancy is induced by maternal ABA. Here we found that AtPER1, a seed-specific peroxiredoxin, is involved in enhancing primary seed dormancy. Two loss-of-function atper1 mutants, atper1-1 and atper1-2, displayed suppressed primary seed dormancy accompanied with reduced ABA and increased GA contents in seeds. Furthermore, atper1 mutant seeds were insensitive to abiotic stresses during seed germination. The expression of several ABA catabolism genes (CYP707A1, CYP707A2, and CYP707A3) and GA biosynthesis genes (GA20ox1, GA20ox3, and KAO3) in atper1 mutant seeds was increased compared to wild-type seeds. The suppressed primary seed dormancy of atper1-1 was completely reduced by deletion of CYP707A genes. Furthermore, loss-of-function of AtPER1 cannot enhance the seed germination ratio of aba2-1 or ga1-t, suggesting that AtPER1-enhanced primary seed dormancy is dependent on ABA and GA. Additionally, the level of reactive oxygen species (ROS) in atper1 mutant seeds was significantly higher than that in wild-type seeds. Taken together, our results demonstrate that AtPER1 eliminates ROS to suppress ABA catabolism and GA biosynthesis, and thus improves the primary seed dormancy and make the seeds less sensitive to adverse environmental conditions.

Keywords: Arabidopsis thaliana; 1-cys peroxiredoxin; ABA; AtPER1; ROS; primary seed dormancy.

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References

    1. Aalenf, R.B., Opsahl-Ferstad, H.-G., Linnestad, C. and Olsen, O.-A. (1994) Transcripts encoding an oleosin and a dormancy-related protein are present in both the aleurone layer and the embryo of developing barley (Hordeum-vulgare L.) seeds. Plant J. 5(3), 385-396.
    1. Ali-Rachedi, S., Bouinot, D., Wagner, M.H., Bonnet, M., Sotta, B., Grappin, P. and Jullien, M. (2004) Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana. Planta, 219, 479-488.
    1. Apel, K. and Hirt, H. (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Ann. Rev. Plant Biol. 55, 373-399.
    1. Bailly, C. and Bailly, C. (2004) Active oxygen species and antioxidants in seed biology. Seed Sci. Res. 14, 93-107.
    1. Baskin, J.M. and Baskin, C.C. (2004) A classification system for seed dormancy. Seed Sci. Res. 14, 1-16.

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