Spermine application alleviates salinity induced growth and photosynthetic inhibition in Solanum lycopersicum by modulating osmolyte and secondary metabolite accumulation and differentially regulating antioxidant metabolism

Abstract

Influence of exogenously applied spermine (Spm) on growth and salinity stress tolerance in tomato was investigated. Salinity reduced growth, chlorophyll synthesis and mineral uptake leading to significant reduction in photosynthesis, however Spm application proved beneficial in alleviating the decline to considerable extent. Applied Spm improved nitrate reductase activity, δ-amino levulinic acid content and gas exchange parameters more apparently at 100 μM than 50 μM concentrations. Spm application enhanced the accumulation of compatible osmolytes including proline, glycine betaine and sugars leading to greater tissue water content and photosynthesis. Salinity stress induced oxidative effects were mitigated by Spm treatment reflected interms of reduced accumulation of reactive oxygen species and the activities of protease and lipoxygenase, hence leading to membrane strengthening and protection of their function. Differential influence of exogenous Spm was evident on the functioning of antioxidant system with SOD, GR and APX activities much higher in Spm treated seedlings than CAT and DHAR. Increased synthesis of GSH, AsA and tocopherol in Spm treated seedlings was obvious thereby helping in maintaining the redox homeostasis and the enzymatic antioxidant functioning. Interestingly Spm application maintained the nitric oxide levels higher than control under normal condition while as lowered its concentrations in salinity stressed seedlings depicting existence of probable interaction. Activities of polyamine metabolizing enzymes was up-regulated and the accumulation of secondary metabolites including phenols and flavonoids also increased due to Spm application. Further studies are required to understand the mechanisms clearly.

Keywords: Antioxidant system; Lipoxygenase; Oxidative stress; Protease; Secondary metabolites; Solanum lycopersicum; Spermine.